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Information Journal Paper

Title

AMMONIUM TO NITRATE RATIO AFFECTS PROTOCORM LIKE BODIES PLB FORMATION IN VITRO OF HYBRID CYMBIDIUM

Pages

  155-160

Keywords

PLB 
TEIXEIRA CYMBIDIUM (TC) MEDIUM 

Abstract

 Carbon and nitrogen are the two elements that most affect plant organogenesis.In vitro, usually as part of the macronutrients. Some anecdotal evidence from the literature suggests that the AMMONIUM (NH4+) to NITRATE (NO3–) ratio may affect orchid organogenesis. In this study, to test this hypothesis, different NH4+: NO3– ratios were tested on the development of protocorm-like bodies (PLBs) of hybrid Cymbidium Twilight Moon ‘Day Light’, when modified in Teixeira Cymbidium (TC) No.1 medium without plant growth regulators. More PLBs formed per half-PLB or per PLB tTCL when TC medium was used while TC from which PGRs or NO3–or NH4+ were removed (two controls) contained significantly less PLBs/explant. The same trend was observed forneo -PLB (i.e., newly formed PLB) fresh weight. A high NO3– ratio favoured neo -PLB formation, but always less than TC medium, but significantly higher than the two controls. tTCLs performed more poorly (i.e., fewer neo-PLBs/explant and lower fresh weight) than half-PLBs. The NH4+: NO3– ratio can be manipulated to increase the level of PLB production in the absence of plant growth regulators.

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    Cite

    APA: Copy

    TEIXEIRA DA SILVA, JAIME A.. (2013). AMMONIUM TO NITRATE RATIO AFFECTS PROTOCORM LIKE BODIES PLB FORMATION IN VITRO OF HYBRID CYMBIDIUM. JOURNAL OF ORNAMENTAL PLANTS (JOURNAL OF ORNAMENTAL AND HORTICULTURAL PLANTS), 3(3), 155-160. SID. https://sid.ir/paper/609281/en

    Vancouver: Copy

    TEIXEIRA DA SILVA JAIME A.. AMMONIUM TO NITRATE RATIO AFFECTS PROTOCORM LIKE BODIES PLB FORMATION IN VITRO OF HYBRID CYMBIDIUM. JOURNAL OF ORNAMENTAL PLANTS (JOURNAL OF ORNAMENTAL AND HORTICULTURAL PLANTS)[Internet]. 2013;3(3):155-160. Available from: https://sid.ir/paper/609281/en

    IEEE: Copy

    JAIME A. TEIXEIRA DA SILVA, “AMMONIUM TO NITRATE RATIO AFFECTS PROTOCORM LIKE BODIES PLB FORMATION IN VITRO OF HYBRID CYMBIDIUM,” JOURNAL OF ORNAMENTAL PLANTS (JOURNAL OF ORNAMENTAL AND HORTICULTURAL PLANTS), vol. 3, no. 3, pp. 155–160, 2013, [Online]. Available: https://sid.ir/paper/609281/en

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