STUDY ON MATING TYPES AND SENSITIVITY TO STROBILURIN FUNGICIDE IN FUNGAL WHEAT PATHOGENMYCOSPHAERELLA GRAMINICOLA

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SAIDI ABBAS; SAFAIE NASER; PALIZI PARISA

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Journal: JOURNAL OF CROP PROTECTION Year:2012 | Volume:1 | Issue:4 Page(s): 271-277

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Title

STUDY ON MATING TYPES AND SENSITIVITY TO STROBILURIN FUNGICIDE IN FUNGAL WHEAT PATHOGENMYCOSPHAERELLA GRAMINICOLA

Author(s)

SAIDI ABBAS | SAFAIE NASER | PALIZI PARISA | Issue Writer Certificate

Keywords

MAT1-1

STROBILURIN

ZYMOSEPTORIA TRITICI

RESISTANCE

Abstract

Mycosphaerella graminicola, the causal agent of septoria tritici blotch (STB), is a widespread and significant pathogen of wheat. To determine mating types, 89 isolates were collected randomly from wheat fields of Khuzestan, East Azerbaijan, Ardebil, Kermanshah and Golestan provinces of Iran, during 2006-7.DNA was extracted based on standard protocols. Multiplex PCR was conducted using two pairs of mating type-specific primers forMAT1-1 and MAT1-2.Sensitivity to STROBILURIN fungicide was determined using strobSNPrc7 and strobSNPrc1 primers. The results showed that in 35 isolates, a fragment of 340 bp amplified withMAT1-1 idiomorph specific primers and in 54 isolates a fragment of 660 bp was amplified withMAT1-2 idiomorph specific primers.While the mating type frequencies were highly unequal, theMAT1-1 was predominant. All isolates were sensitive to STROBILURIN and amplified a fragment of 639 bp. It is concluded that both mating types are present in Iran, although with different frequencies, which may affect genetic variation through sexual cycle. Meanwhile the studied isolates were not resistant to STROBILURIN fungicides which may be due to growing wheat cultivars resistant to STB rather than using STROBILURIN fungicides as a dominant control method.

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APA: Copy

SAIDI, ABBAS, SAFAIE, NASER, & PALIZI, PARISA. (2012). STUDY ON MATING TYPES AND SENSITIVITY TO STROBILURIN FUNGICIDE IN FUNGAL WHEAT PATHOGENMYCOSPHAERELLA GRAMINICOLA. JOURNAL OF CROP PROTECTION, 1(4), 271-277. SID. https://sid.ir/paper/616390/en

Vancouver: Copy

SAIDI ABBAS, SAFAIE NASER, PALIZI PARISA. STUDY ON MATING TYPES AND SENSITIVITY TO STROBILURIN FUNGICIDE IN FUNGAL WHEAT PATHOGENMYCOSPHAERELLA GRAMINICOLA. JOURNAL OF CROP PROTECTION[Internet]. 2012;1(4):271-277. Available from: https://sid.ir/paper/616390/en

IEEE: Copy

ABBAS SAIDI, NASER SAFAIE, and PARISA PALIZI, “STUDY ON MATING TYPES AND SENSITIVITY TO STROBILURIN FUNGICIDE IN FUNGAL WHEAT PATHOGENMYCOSPHAERELLA GRAMINICOLA,” JOURNAL OF CROP PROTECTION, vol. 1, no. 4, pp. 271–277, 2012, [Online]. Available: https://sid.ir/paper/616390/en

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