USE OF N-TRIMETHYL CHITOSAN FOR INTRANASAL DELIVERY OF DNA ENCODING M2E-HSP70C IN MICE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

DABAGHIAN M.; EBRAHIMI S.M.; NIKBAKHT GH.R.; TEBIANIAN M.; REZAEI MOKARAM A.; IMAN M.; TAVANGAR RANJBAR A.R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Veterinary Medicine Year:2013 | Volume:7 | Issue:2 Page(s): 123-128

Download Full-Text

View:

297

Download:

Cites:

Information Journal Paper

Title

USE OF N-TRIMETHYL CHITOSAN FOR INTRANASAL DELIVERY OF DNA ENCODING M2E-HSP70C IN MICE

Author(s)

Keywords

INFLUENZA

M2E

HSP70

N-TRIMETHYL CHITOSAN (TMC)

INTRA NASAL DELIVERY

Abstract

BACKGROUND: INFLUENZA outbreak has become a great lifethreatening disease in the world. Nasal vaccines can induce systemic IgG and mucosal IgA antibody responses, which establish two layers of immune defense against the infectious pathogens like INFLUENZA. Mucosal vaccines must overcome several limitations, including the mucociliary clearance and inefficient uptake of soluble antigens. Therefore, nasal vaccines require potent adjuvants and delivery systems.OBJECTIVES: In this study we evaluated the effect of N-trimethyl chitosan (TMC) as a potent vehicle for DNA encoding M2E/HSP70c in order for intranasal administration in mice.METHODS: Ectodomain of the conserved INFLUENZA matrix protein 2 (M2e), which has been found to induce heterosubtypic immunity, was fused to HSP70359-610 or C-terminus of Mycobacterium tuberculosis HSP70 (HSP70c) in pcDNA3.1 vector (pcDNA/M2e-HSP70c) and then encapsulated into a derivative of chitosan, N-trimethyl chitosan (TMC). After encapsulation of the plasmid, physical properties of the particles were investigated using ZetasizerÒ 3000 the particles were then administered through the intranasal delivery in BALB/c mice.RESULTS: It was found that the particles had a size ranging between 90-120nm and positive surface charge. The intranasal immunization with M2E- HSP70c+TMC in BALB/c mice significantly induced higher M2E specific IgG than those induced in control groups (pcDNA/M2e-HSP70c without TMC, pcDNA/M2e, bearing M2E alone, and PBS).CONCLUSIONS: The present study showed that the encapsulation of M2E/ HSP70c into N-trimethyl chitosan (TMC) could strongly induce the humoral immune response against the M2E-HSP70c plasmid without lowering the adjuvant efficacy of HSP70c.

Cites

No record.

References

No record.

Cite

APA: Copy

DABAGHIAN, M., EBRAHIMI, S.M., NIKBAKHT, GH.R., TEBIANIAN, M., REZAEI MOKARAM, A., IMAN, M., & TAVANGAR RANJBAR, A.R.. (2013). USE OF N-TRIMETHYL CHITOSAN FOR INTRANASAL DELIVERY OF DNA ENCODING M2E-HSP70C IN MICE. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), 7(2), 123-128. SID. https://sid.ir/paper/618234/en

Vancouver: Copy

DABAGHIAN M., EBRAHIMI S.M., NIKBAKHT GH.R., TEBIANIAN M., REZAEI MOKARAM A., IMAN M., TAVANGAR RANJBAR A.R.. USE OF N-TRIMETHYL CHITOSAN FOR INTRANASAL DELIVERY OF DNA ENCODING M2E-HSP70C IN MICE. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH)[Internet]. 2013;7(2):123-128. Available from: https://sid.ir/paper/618234/en

IEEE: Copy

M. DABAGHIAN, S.M. EBRAHIMI, GH.R. NIKBAKHT, M. TEBIANIAN, A. REZAEI MOKARAM, M. IMAN, and A.R. TAVANGAR RANJBAR, “USE OF N-TRIMETHYL CHITOSAN FOR INTRANASAL DELIVERY OF DNA ENCODING M2E-HSP70C IN MICE,” IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), vol. 7, no. 2, pp. 123–128, 2013, [Online]. Available: https://sid.ir/paper/618234/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops

No record.