AN OPTIMIZED AFFORDABLE DNA-EXTRACTION METHOD FROM SALMONELLA ENTERICA ENTERITIDIS FOR PCR EXPERIMENTS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

KARIMNASAB N.; TADAYON K.; KHAKI P.; MORADI BIDHENDI S.; GHADERI R.; SEKHAVATI M.; ASADI F.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: ARCHIVES OF RAZI INSTITUTE Year:2013 | Volume:68 | Issue:2 Page(s): 105-109

Download Full-Text

View:

581

Download:

290

Cites:

Information Journal Paper

Title

AN OPTIMIZED AFFORDABLE DNA-EXTRACTION METHOD FROM SALMONELLA ENTERICA ENTERITIDIS FOR PCR EXPERIMENTS

Author(s)

Keywords

DNA EXRTACTION

SALMONELLA ENTERICA ENTERITIDIS

STM2

DNASE

PROTEINASE K

Abstract

In diagnostic and research bacteriology settings with budget and staff restrictions, fast and cost-effective genome extraction methods are desirable. If not inactivated properly, cellular and/or environmental DNA nucleases will degrade genomic material during the extraction stage, and therefore might give rise to incorrect results in PCR experiments. When crude cell extracts, PROTEINASE K-treated templates and purified DNAs prepared by phenol-chloroform-isoamylalcohol method as well as a commercial extraction kit were subjected to the SALMONELLA ENTERICA ENTERITIDIS specific STM2 PCR, with exception of crude cell extract, PCR products from all other three methods saved their integrity for 28 days post-generation. This work aimed to find out whether improvement to boiling method can guaranty stability of PCR products. As results showed, treatment of crude cell extracts from S. Enteritidis with PROTEINASE K offers an inexpensive, fast and effective DNA extraction method suitable for high-throughput laboratories.

Cites

EVALUATE THE PREVALENCE OF AAC (6’)-IE-APH(2’’)-IA GENE AMONG CLINICAL ISOLATES OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS ISOLATES FROM THE HOSPITAL MASHHAD

References

No record.

Cite

APA: Copy

KARIMNASAB, N., TADAYON, K., KHAKI, P., MORADI BIDHENDI, S., GHADERI, R., SEKHAVATI, M., & ASADI, F.. (2013). AN OPTIMIZED AFFORDABLE DNA-EXTRACTION METHOD FROM SALMONELLA ENTERICA ENTERITIDIS FOR PCR EXPERIMENTS. ARCHIVES OF RAZI INSTITUTE, 68(2), 105-109. SID. https://sid.ir/paper/619778/en

Vancouver: Copy

KARIMNASAB N., TADAYON K., KHAKI P., MORADI BIDHENDI S., GHADERI R., SEKHAVATI M., ASADI F.. AN OPTIMIZED AFFORDABLE DNA-EXTRACTION METHOD FROM SALMONELLA ENTERICA ENTERITIDIS FOR PCR EXPERIMENTS. ARCHIVES OF RAZI INSTITUTE[Internet]. 2013;68(2):105-109. Available from: https://sid.ir/paper/619778/en

IEEE: Copy

N. KARIMNASAB, K. TADAYON, P. KHAKI, S. MORADI BIDHENDI, R. GHADERI, M. SEKHAVATI, and F. ASADI, “AN OPTIMIZED AFFORDABLE DNA-EXTRACTION METHOD FROM SALMONELLA ENTERICA ENTERITIDIS FOR PCR EXPERIMENTS,” ARCHIVES OF RAZI INSTITUTE, vol. 68, no. 2, pp. 105–109, 2013, [Online]. Available: https://sid.ir/paper/619778/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops

No record.