مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

CLONING

Pages

  275-277

Keywords

Not Registered.

Abstract

 How can we clone PCR products.pGEMâ‐T Easy Vector System is a convenient system for the cloning of PCR products.How is the vector prepared?The vector is prepared by cutting the pGEMâ‐T Easy Vector with EcoRV and adding a 3' terminal thymidine to both ends (Fig. I). These single 3' T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmids by preventing recircularization of the vector and providing a compatible overhang for PCR products generated by Taq as a thermostable polymerase. Taq polymerase often adds a single deoxyadenosine, in a template‐independent fashion to the 3' ends of the significant proportion of amplified fragments as shown in the Figure. Using this method, only one insert will be ligated into the vector as opposed to multiple insertions that can occur with blunt ended cloning. In addition, with T vector cloning there is no need to dephosphorylate the vector, and there is a low background of relegated vector.

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    • Cite

    APA: Copy

    SHAHHOSSEINI, FATIMA. (2011). CLONING. IRANIAN JOURNAL OF PLANT PHYSIOLOGY, 1(3), 275-277. SID. https://sid.ir/paper/625179/en

    Vancouver: Copy

    SHAHHOSSEINI FATIMA. CLONING. IRANIAN JOURNAL OF PLANT PHYSIOLOGY[Internet]. 2011;1(3):275-277. Available from: https://sid.ir/paper/625179/en

    IEEE: Copy

    FATIMA SHAHHOSSEINI, “CLONING,” IRANIAN JOURNAL OF PLANT PHYSIOLOGY, vol. 1, no. 3, pp. 275–277, 2011, [Online]. Available: https://sid.ir/paper/625179/en

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