EFFICIENT PROTOCOL FOR PROTOPLAST ISOLATION AND PLANT REGENERATION OF FRITILLARIA IMPERIALIS L.

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CHAMANI E.; TAHAMI S.K.

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Journal: JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST) Year:2016 | Volume:18 | Issue:2 Page(s): 467-482

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Title

EFFICIENT PROTOCOL FOR PROTOPLAST ISOLATION AND PLANT REGENERATION OF FRITILLARIA IMPERIALIS L.

Author(s)

CHAMANI E. | TAHAMI S.K. | Issue Writer Certificate

Keywords

CALLUS FORMATIONQ3

MEDIUMQ3

PROTOPLAST CULTUREQ3

VIABILITYQ3

Abstract

The present study reports an efficient protocol for isolation and regeneration of protoplasts from callus of Fritillaria imperialis L. There is no published method recommended for protoplast isolation and regeneration fromFritillaria imperialis L. A range of factors, which influence the success of isolation and regeneration of F. imperialis protoplasts, were investigated. From the results obtained, callus Fresh Weight (FW) of 0.4 g produced the highest number of viable protoplasts, which was 1.12×105 protoplasts g-1 FW. The highest amount of viable protoplasts (1.01×105 protoplasts g-1 FW) was obtained when the mannitol concentration was maintained at 9% (w/v). The best treatment for isolation of F. imperialis protoplast (1.37×105 protoplasts g-1 FW) was treatment with 2% cellulase and 0.1% pectinase with 9% mannitol for 8 h. For enhancement of the protoplasts division and the percentage of colony formation, different concentrations from Casein Hydrolysate (CH), 2, 4-Dichlorophenoxyacetic acid (2, 4-D) and Benzyl-Adenine (BA) were used. The results revealed that cell wall and colony formation were better in liquid MEDIUM than those on semi-solid MEDIUM. The highest plating efficiency (1.26×106 per g FW) and highest CALLUS FORMATION were obtained using the MEDIUM containing 0.5 mg L-1 2, 4-D, 1 mg L-1 BA, and 200 mg L-1 CH. Micro-calli were formed after one month of culture. Many plantlets were formed on the calli after transfer of the proliferated calli to regeneration MEDIUM. The highest plantlet regeneration (100%) was obtained using the MEDIUM containing 0.5 mg L–1 (Naphthalene Acetic Acid) NAA and 1.5 mg L-1 BA.

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APA: Copy

CHAMANI, E., & TAHAMI, S.K.. (2016). EFFICIENT PROTOCOL FOR PROTOPLAST ISOLATION AND PLANT REGENERATION OF FRITILLARIA IMPERIALIS L.. JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST), 18(2), 467-482. SID. https://sid.ir/paper/62707/en

Vancouver: Copy

CHAMANI E., TAHAMI S.K.. EFFICIENT PROTOCOL FOR PROTOPLAST ISOLATION AND PLANT REGENERATION OF FRITILLARIA IMPERIALIS L.. JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST)[Internet]. 2016;18(2):467-482. Available from: https://sid.ir/paper/62707/en

IEEE: Copy

E. CHAMANI, and S.K. TAHAMI, “EFFICIENT PROTOCOL FOR PROTOPLAST ISOLATION AND PLANT REGENERATION OF FRITILLARIA IMPERIALIS L.,” JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST), vol. 18, no. 2, pp. 467–482, 2016, [Online]. Available: https://sid.ir/paper/62707/en

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