EFFECT OF FRESH AND FROZEN SEMEN ON IN VITRO FERTILIZATION AND SUBSEQUENT DEVELOPMENT OF GOAT EMBRYOS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SAHA S.; KHANDOKER M.A.M.Y.; ASAD L.Y.; REZA A.M.M.T.; HOQUE A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE Year:2014 | Volume:4 | Issue:2 Page(s): 325-330

Download Full-Text

View:

329

Download:

176

Cites:

Information Journal Paper

Title

EFFECT OF FRESH AND FROZEN SEMEN ON IN VITRO FERTILIZATION AND SUBSEQUENT DEVELOPMENT OF GOAT EMBRYOS

Author(s)

Keywords

EMBRYO

FRESH SEMEN

FROZEN SEMEN

IN VITRO FERTILIZATION

Abstract

This study was undertaken to compare the efficiency of fresh and frozen-thawed buck semen on IN VITRO FERTILIZATION (IVF) of goat oocytes. Cumulus oocytes complexes (COCs) were collected by aspiration of 2-6 mm diameter follicles, which were obtained from slaughterhouse. Upon grading, only normal quality COCs were maturated in TCM-199 for 48 hours. The percentage of COCs reached to the M-II stage was 61.41±1.97%. The matured COCs were fertilized for 5 hours in Brackett and Oliphant media using fresh and frozen thawed semen separately. After fertilization the oocytes were cultured in TCM-199 for 48 hours to observe the cleavage rate. The maturation, fertilization and culture were performed in an incubator at 38.5oC with 5% CO2 in humidified air. After fertilization cleavage rates were observed to check the fitness of zygotes to be morula and blastocyst. It was observed that the rates of normal fertilization (2 PN formations) for fresh and FROZEN SEMEN were 36.02±2.79 and 34.73±2.58%, respectively and the cleavage rates were 25.19±2.5 and 21.01±2.8%, respectively. No significant differences (P>0.05) was observed between fresh and FROZEN SEMEN in the efficiency of IN VITRO FERTILIZATION and subsequent development of goat EMBRYOs. It can be concluded that, both fresh and FROZEN SEMEN can be used for IVF and subsequent development of goat EMBRYOs.

Cites

No record.

References

ROLE OF GELATINASE ON FOLLICULAR ATRESIA IN THE BOVINE OVARY

Cite

APA: Copy

SAHA, S., KHANDOKER, M.A.M.Y., ASAD, L.Y., REZA, A.M.M.T., & HOQUE, A.. (2014). EFFECT OF FRESH AND FROZEN SEMEN ON IN VITRO FERTILIZATION AND SUBSEQUENT DEVELOPMENT OF GOAT EMBRYOS. IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE, 4(2), 325-330. SID. https://sid.ir/paper/629306/en

Vancouver: Copy

SAHA S., KHANDOKER M.A.M.Y., ASAD L.Y., REZA A.M.M.T., HOQUE A.. EFFECT OF FRESH AND FROZEN SEMEN ON IN VITRO FERTILIZATION AND SUBSEQUENT DEVELOPMENT OF GOAT EMBRYOS. IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE[Internet]. 2014;4(2):325-330. Available from: https://sid.ir/paper/629306/en

IEEE: Copy

S. SAHA, M.A.M.Y. KHANDOKER, L.Y. ASAD, A.M.M.T. REZA, and A. HOQUE, “EFFECT OF FRESH AND FROZEN SEMEN ON IN VITRO FERTILIZATION AND SUBSEQUENT DEVELOPMENT OF GOAT EMBRYOS,” IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE, vol. 4, no. 2, pp. 325–330, 2014, [Online]. Available: https://sid.ir/paper/629306/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops