PROTEIN PATTERNS AND LARVICIDE ACTIVITY OF CRYSTALLINE INCLUSIONS OF BACILLUS THURINGIENSIS SSP. KUMAMOTOENSIS DSM 6070

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KUTASI J.; KOVACS R.; PUSPAN I.; MAKK J.; TAKACS K.; ERDELYI B.; IMRE CS.; KARPATI E.

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Journal: JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST) Year:2016 | Volume:18 | Issue:SUPP. Page(s): 1945-1951

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Title

PROTEIN PATTERNS AND LARVICIDE ACTIVITY OF CRYSTALLINE INCLUSIONS OF BACILLUS THURINGIENSIS SSP. KUMAMOTOENSIS DSM 6070

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Keywords

BACILLUS THURINGIENSISQ3

PARASPORAL CRYSTALSQ3

POLYACRYLAMIDE GEL-ELECTROPHORESISQ3

SCANNING ELECTRON MICROSCOPYQ3

Abstract

Morphological and electrophoretic analysis of the crystalline inclusions (parasporal crystals) of sporulated cultures ofB. thuringiensis ssp. kumamotoensis DSM 6070 (Bt 6070) was conducted via phase contrast and SCANNING ELECTRON MICROSCOPY and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The activity of the spore-crystal suspensions against house fly (Musca domestica) larvae was also assessed. Bipyramidal and smaller, irregular shaped crystals were observed in the sporulated cultures.130, 75 and 25 kDa bands were detected in the protein pattern. The presence of 25 kda proteins in Bt6070 has not been reported earlier. The spore-crystal suspension showed significant larvicide activity against housefly larvae. Larvicide activity ofB. thuringiensis ssp.kumamotoensisagainst any dipteran species has not been detected yet. Further studies are needed on identifying the dipteran- active fraction.

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APA: Copy

KUTASI, J., KOVACS, R., PUSPAN, I., MAKK, J., TAKACS, K., ERDELYI, B., IMRE, CS., & KARPATI, E.. (2016). PROTEIN PATTERNS AND LARVICIDE ACTIVITY OF CRYSTALLINE INCLUSIONS OF BACILLUS THURINGIENSIS SSP. KUMAMOTOENSIS DSM 6070. JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST), 18(SUPP.), 1945-1951. SID. https://sid.ir/paper/62981/en

Vancouver: Copy

KUTASI J., KOVACS R., PUSPAN I., MAKK J., TAKACS K., ERDELYI B., IMRE CS., KARPATI E.. PROTEIN PATTERNS AND LARVICIDE ACTIVITY OF CRYSTALLINE INCLUSIONS OF BACILLUS THURINGIENSIS SSP. KUMAMOTOENSIS DSM 6070. JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST)[Internet]. 2016;18(SUPP.):1945-1951. Available from: https://sid.ir/paper/62981/en

IEEE: Copy

J. KUTASI, R. KOVACS, I. PUSPAN, J. MAKK, K. TAKACS, B. ERDELYI, CS. IMRE, and E. KARPATI, “PROTEIN PATTERNS AND LARVICIDE ACTIVITY OF CRYSTALLINE INCLUSIONS OF BACILLUS THURINGIENSIS SSP. KUMAMOTOENSIS DSM 6070,” JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST), vol. 18, no. SUPP., pp. 1945–1951, 2016, [Online]. Available: https://sid.ir/paper/62981/en

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