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Information Journal Paper

Title

ANALYSIS OF SALINE ACTIVATOR SOLUTION EFFECTS ON SPERM QUALITY INDICES OF BARBUS SHARPEYI BY IMAGE J SOFTWARE

Pages

  357-377

Abstract

 Regarding low FERTILIZATION rate of BARBUS SHARPEYI, one of the main Barbus species in south of IRAN, SALINE ACTIVATOR SOLUTIONs (SAS 1 and 2) were used for increasing of SPERM activation and its SPERM quality indices were assessed by using Image J software as a fish SPERM adapted tools. SAS1 (45mM NaCl, 5mM KCl, 30mM Tris, 188±2.3 mOsmol/kg) and SAS2 (50mM NaCl, 30mM KCl, 30mM Tris, 189±1.7 mOsmol/kg) were added to a mixture of 12 male semen comparing with distilled and tap water and their effects on VCL, VSL, ALH, Linearity, %motility, SPERMatozoa path, % A, B and C SPERMatozoa type, FERTILIZATION, hatching and deformity rate were analyzed in triplicate. Results showed that SAS2 had the highest VCL (21.37±0.36 mm/s), VSL (11.6±0.3 mm/s) and motility percentage (85.75±1.56%) among treatments. The highest A type SPERMatozoa percentage was recorded for SAS1 (47.16±3.15%) and SAS2 (43.89±4.54%). The lowest C type SPERMatozoa percentage was recorded using SAS2 (14.24±1.56 %). Also, the highest FERTILIZATION rate was achieved from SAS2 (79.5±0.37%) and SAS1 (77.74±4%) respectively. Furthermore the lowest larval deformity rate (13.77±1.03%) was shown due to the use of SAS2. Final conclusion confirmed that SAS1 and SAS2 have positive and significant effects (P<0.05) on extending the time length of SPERMs movement, VCL and VSL of B. sharpeyi which consequently resulted in better efficiency of propagation of this species in south of IRAN.

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    APA: Copy

    KALBASSI, M.R., LORESTANI, R., & GHAFLE MARAMAZI, J.. (2013). ANALYSIS OF SALINE ACTIVATOR SOLUTION EFFECTS ON SPERM QUALITY INDICES OF BARBUS SHARPEYI BY IMAGE J SOFTWARE. IRANIAN JOURNAL OF FISHERIES SCIENCES, 12(2), 357-377. SID. https://sid.ir/paper/630150/en

    Vancouver: Copy

    KALBASSI M.R., LORESTANI R., GHAFLE MARAMAZI J.. ANALYSIS OF SALINE ACTIVATOR SOLUTION EFFECTS ON SPERM QUALITY INDICES OF BARBUS SHARPEYI BY IMAGE J SOFTWARE. IRANIAN JOURNAL OF FISHERIES SCIENCES[Internet]. 2013;12(2):357-377. Available from: https://sid.ir/paper/630150/en

    IEEE: Copy

    M.R. KALBASSI, R. LORESTANI, and J. GHAFLE MARAMAZI, “ANALYSIS OF SALINE ACTIVATOR SOLUTION EFFECTS ON SPERM QUALITY INDICES OF BARBUS SHARPEYI BY IMAGE J SOFTWARE,” IRANIAN JOURNAL OF FISHERIES SCIENCES, vol. 12, no. 2, pp. 357–377, 2013, [Online]. Available: https://sid.ir/paper/630150/en

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