مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

585
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

172
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

DETECTION OF THE EAEA GENE IN ESCHERICHIA COLI ISOLATED FROM BROILER CHICKENS BY POLYMERASE CHAIN REACTION

Author(s)

 ZAKERI A. | Issue Writer Certificate 

Pages

  417-420

Abstract

 The aim of this study was to isolate ESCHERICHIA COLI from chickens and to determine the presence of the EAEA GENE, a virulence factor detected in ESCHERICHIA COLI, in the isolates by polymerase chain reaction (PCR). Different chicken organs (lung, liver and spleen) were inoculated onto blood agar and biochemical tests were performed on the suspicious isolates. ESCHERICHIA COLI was isolated from 67.5% (81.120) of the samples. DNA was extracted from these isolates and was amplified by PCR, using a pair of primers derived from the eaeA (virulence) gene. In the agarose gel examination of PCR products, 48.1% (39.81) of the isolates were determined to have this virulence factor. Correct amplification with a molecular length of 384 bp was obtained in the analysis of all the isolates by species-specific PCR, which confirmed the results of the detection of the EAEA GENE in ESCHERICHIA COLI. The EAEA GENE, which is mainly responsible for the virulence of ESCHERICHIA COLI, is commonly present in ESCHERICHIA COLI strains isolated from this region and the significance of this situation for animal and public health was discussed.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    ZAKERI, A.. (2014). DETECTION OF THE EAEA GENE IN ESCHERICHIA COLI ISOLATED FROM BROILER CHICKENS BY POLYMERASE CHAIN REACTION. IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE, 4(2), 417-420. SID. https://sid.ir/paper/644501/en

    Vancouver: Copy

    ZAKERI A.. DETECTION OF THE EAEA GENE IN ESCHERICHIA COLI ISOLATED FROM BROILER CHICKENS BY POLYMERASE CHAIN REACTION. IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE[Internet]. 2014;4(2):417-420. Available from: https://sid.ir/paper/644501/en

    IEEE: Copy

    A. ZAKERI, “DETECTION OF THE EAEA GENE IN ESCHERICHIA COLI ISOLATED FROM BROILER CHICKENS BY POLYMERASE CHAIN REACTION,” IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE, vol. 4, no. 2, pp. 417–420, 2014, [Online]. Available: https://sid.ir/paper/644501/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops





    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button