INVESTIGATION OF (STEAROYL-COA DESATURASE 1) SCD1 GENE POLYMORPHISM IN KHUZESTAN BUFFALO POPULATION USING PCR-RFLP METHOD

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TAGHIZADEH K.; BEIGI NASIRI M.T.; FAYAZI J.; BUJARPOOR M.

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Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE Year:2014 | Volume:4 | Issue:2 Page(s): 425-428

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Title

INVESTIGATION OF (STEAROYL-COA DESATURASE 1) SCD1 GENE POLYMORPHISM IN KHUZESTAN BUFFALO POPULATION USING PCR-RFLP METHOD

Author(s)

TAGHIZADEH K. | BEIGI NASIRI M.T. | FAYAZI J. | BUJARPOOR M. | Issue Writer Certificate

Keywords

BUFFALO

HARDY-WEINBERG EQUILIBRIUM

MUFA

PCR-RFLP

SCD1 GENE

Abstract

Stearoyl-CoA desaturase (SCD) is a rate-limiting enzyme in the biosynthesis of monounsaturated fatty ac-ids (MUFA). A number of studies support the hypothesis that SCD gene regulation and polymorphism may affect fatty acid composition and fat quality in meat and milk. Single nucleotide polymorphisms in the cod-ing region of the bovine stearoyl-CoA desaturase gene have been predicted to result in p. 293A (alanine at amino acid 293) and p. 293V (valine at amino acid 293) alleles at the stearoyl-CoA desaturase1 locus. The objective of this study was to characterize polymorphisms of stearoyl-CoA desaturase 1 (SCD1) gene in Khuzestan BUFFALO population. This is the first study of SCD1 GENE polymorphism in Iranian BUFFALO. The blood samples were taken from 85 BUFFALO of Khuzestan-Iran population. Genomic DNA was extracted using a kit DIAtom DNA prep. After this, PCR reactions were made by using primers that encloses exon V. A 400 bp fragment amplified with standard PCR method. The PCR products were digested by NCO1 restriction enzyme with PCR-RFLP method. All samples were genotyped on 3% agarose gels stained with ethidium bromide. Electrophoretic mobility shift determined that there were not genetic differences between these animals in the studied region by using NCOI enzyme. Also, this study showed that genotype frequencies were not in HARDY-WEINBERG EQUILIBRIUM for SCD1 GENE.

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APA: Copy

TAGHIZADEH, K., BEIGI NASIRI, M.T., FAYAZI, J., & BUJARPOOR, M.. (2014). INVESTIGATION OF (STEAROYL-COA DESATURASE 1) SCD1 GENE POLYMORPHISM IN KHUZESTAN BUFFALO POPULATION USING PCR-RFLP METHOD. IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE, 4(2), 425-428. SID. https://sid.ir/paper/647049/en

Vancouver: Copy

TAGHIZADEH K., BEIGI NASIRI M.T., FAYAZI J., BUJARPOOR M.. INVESTIGATION OF (STEAROYL-COA DESATURASE 1) SCD1 GENE POLYMORPHISM IN KHUZESTAN BUFFALO POPULATION USING PCR-RFLP METHOD. IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE[Internet]. 2014;4(2):425-428. Available from: https://sid.ir/paper/647049/en

IEEE: Copy

K. TAGHIZADEH, M.T. BEIGI NASIRI, J. FAYAZI, and M. BUJARPOOR, “INVESTIGATION OF (STEAROYL-COA DESATURASE 1) SCD1 GENE POLYMORPHISM IN KHUZESTAN BUFFALO POPULATION USING PCR-RFLP METHOD,” IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE, vol. 4, no. 2, pp. 425–428, 2014, [Online]. Available: https://sid.ir/paper/647049/en

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