مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

309
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

222
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

STUDY OF GENETIC DIVERSITY OF WILD CASPIAN TROUT SALMO TRUTTA CASPIUS IN THE SARDABRUD AND ASTARA RIVERS, USING D- LOOP REGION SEQUENCING

Pages

  352-365

Abstract

 In this study the GENETIC DIVERSITY of wild Caspian trout (Salmo trutta caspius) in the Sardabroud and ASTARA Rivers was evaluated using D- Loop region SEQUENCING. For this purpose, 35 specimens of adult Caspian brown trout were collected from these rivers in the Mazandarn and Gilan Provinces in fall and winter 2011. Approximately 3-5 g of soft and fresh fin tissue was isolated and fixed in ethanol 96% and then transferred to the Caspian Sea Ecology Research Center Genetics Laboratory in Sari, Iran. Genomic DNA from the samples was extracted using Ammonium Acetate Method. The quality and quantity of the extracted DNA were assessed by spectrophotometer and agarose gel (1%) electrophoresis. Polymerase Chain Reaction (PCR) was performed on the target DNA using a primers sequence D- Loop region of mtDNA molecule. Then the product was purified and DNA SEQUENCING was carried out using chain termination method. The D- Loop region of Caspian trout contained 654 bp. Data were analyzed using Bio-Edit, DnaSP, Arlequin and Mega software.20 and 15 haplotypes was observed in SARDABRUD and ASTARA River. Tthe DNA sequence of one of them was recorded in Gene Bank with numbers KC991027 and KF015727.223 and 240 polymorphic loci were detected in SARDABRUD and ASTARA River that all of them were out of Hardy- Weinberg equilibrium (p<0.05). Average nucleotide and haplotype diversity were 0.127±0.067, 1.000±0.005 in SARDABRUD River and 0.118±0.063 and 1.000±0.005 in ASTARA River.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    NAJJAR LASHGARI, S., REZVNI GILKOLAEI, S., KAMALI, A., & SOLTANI, M.. (2017). STUDY OF GENETIC DIVERSITY OF WILD CASPIAN TROUT SALMO TRUTTA CASPIUS IN THE SARDABRUD AND ASTARA RIVERS, USING D- LOOP REGION SEQUENCING. IRANIAN JOURNAL OF FISHERIES SCIENCES, 16(1), 352-365. SID. https://sid.ir/paper/691572/en

    Vancouver: Copy

    NAJJAR LASHGARI S., REZVNI GILKOLAEI S., KAMALI A., SOLTANI M.. STUDY OF GENETIC DIVERSITY OF WILD CASPIAN TROUT SALMO TRUTTA CASPIUS IN THE SARDABRUD AND ASTARA RIVERS, USING D- LOOP REGION SEQUENCING. IRANIAN JOURNAL OF FISHERIES SCIENCES[Internet]. 2017;16(1):352-365. Available from: https://sid.ir/paper/691572/en

    IEEE: Copy

    S. NAJJAR LASHGARI, S. REZVNI GILKOLAEI, A. KAMALI, and M. SOLTANI, “STUDY OF GENETIC DIVERSITY OF WILD CASPIAN TROUT SALMO TRUTTA CASPIUS IN THE SARDABRUD AND ASTARA RIVERS, USING D- LOOP REGION SEQUENCING,” IRANIAN JOURNAL OF FISHERIES SCIENCES, vol. 16, no. 1, pp. 352–365, 2017, [Online]. Available: https://sid.ir/paper/691572/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button