MOLECULAR CHARACTERIZATION OF APOLIPOPROTEIN A-I FROM THE SKIN MUCOSA OF CYPRINUS CARPIO

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JOLODAR A.

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Journal: Iranian Journal of Fisheries Sciences Year:2017 | Volume:16 | Issue:1 Page(s): 366-381

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Title

MOLECULAR CHARACTERIZATION OF APOLIPOPROTEIN A-I FROM THE SKIN MUCOSA OF CYPRINUS CARPIO

Author(s)

JOLODAR A. | Issue Writer Certificate

Keywords

APOLIPOPROTEIN A-I

COMMON CARP

CYPRINUS CARPIO

EPIDERMAL MUCUS

FULL-LENGTH SEQUENCE

Abstract

APOLIPOPROTEIN A-I is the most abundant protein inCyprinus carpio plasma that plays an important role in lipid transport and protection of the skin by means of its antimicrobial activity. A 527 bp cDNA fragment encoding C terminus part of apoA-I from the skin mucosa of COMMON CARP was isolated using RT-PCR. After GenBank database searching, a partial sequence containing a coding sequence (CDS) relating to this gene was found. Overlapping of the cDNA fragment with this CDS allowed us to obtain the FULL-LENGTH SEQUENCE including non-coding regions. This sequence has 1170bp including a polyA tail of 18 bp plus 45 and 354 bp at the 3' - and 5' -untranslatedregions, respectively. The complete sequence contained an open reading frame of 256 amino containing 5 amino acid propeptides with a predicted molecular mass of 29.967 kDa and theoretical pI of 6.13.The signal peptide of COMMON CARP apoA-I was predicted to have the most likely cleavage site between amino acid positions 17 and 18. Domain analysis of COMMON CARP apoA-I showed the conserved domain of Apolipoprotein A1/A4/E between amino acid resides 67 to 251. The similarity search indicated that COMMON CARP apoA-I matched apoA protein from the group of fish with 45-77% similarity, but showed relatively low levels of similarity to its mammalian counterparts (20-28%).It was shown that the secondary structure ofC.carpioapoA-I consisted of a-helical predominantly amphipathic in nature and was characterized by the presence of thirteen conserved repeats.

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APA: Copy

JOLODAR, A.. (2017). MOLECULAR CHARACTERIZATION OF APOLIPOPROTEIN A-I FROM THE SKIN MUCOSA OF CYPRINUS CARPIO. IRANIAN JOURNAL OF FISHERIES SCIENCES, 16(1), 366-381. SID. https://sid.ir/paper/701481/en

Vancouver: Copy

JOLODAR A.. MOLECULAR CHARACTERIZATION OF APOLIPOPROTEIN A-I FROM THE SKIN MUCOSA OF CYPRINUS CARPIO. IRANIAN JOURNAL OF FISHERIES SCIENCES[Internet]. 2017;16(1):366-381. Available from: https://sid.ir/paper/701481/en

IEEE: Copy

A. JOLODAR, “MOLECULAR CHARACTERIZATION OF APOLIPOPROTEIN A-I FROM THE SKIN MUCOSA OF CYPRINUS CARPIO,” IRANIAN JOURNAL OF FISHERIES SCIENCES, vol. 16, no. 1, pp. 366–381, 2017, [Online]. Available: https://sid.ir/paper/701481/en

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