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Information Journal Paper

Title

ISOLATION AND IDENTIFICATION OF BACTERIA FROM ROTARY DRUM COMPOST OF WATER HYACINTH

Pages

  245-253

Keywords

PCR (POLYMERASE CHAIN REACTION) 

Abstract

 Purpose: The present work aims to isolate and identify bacterial community from the rotary drum compost of green waste such as the water hyacinth. Compost quality was also investigated with the physicochemical parameters and the heavy metal analysis.Methods: For ROTARY DRUM COMPOSTING, a waste mixture of 150 kg with water hyacinth, cow dung and sawdust were prepared in the proportion of 6: 3: 1, respectively. The physicochemical parameters such as pH, temperature, volatile solids and the electrical conductivity were analyzed to detect the compost quality. Pb, Ni, Zn, and Cd were investigated for total HEAVY METALS, toxicity characteristic leaching procedure (TCLP), diethylenetriamine pentaacetic acid (DTPA) and water solubility tests.Consistent and active bacterial community were isolated from the rotary drum compost of water hyacinth. Culture dependent and culture-independent techniques were approached for the isolation process.Results: Twelve bacteria were isolated and identified by 16S rDNA sequencing and phylogenetic analysis; they majorly belonged to the BACILLUS and ENTEROBACTER family.The analysis of temperature, pH, EC, VS and HEAVY METALS depicted the good quality of compost. HEAVY METALS concentration was in Pb[Ni [Zn [Cd; however, for Pb, Cd and Ni water solubility remained non-detectable, DTPA concentration for Pb and Cd was also found to be nondetectable.Conclusions: The microbes in water hyacinth compost are metabolically active degrading the organic matter, surviving in the heavy metal-loaded compost environment. Thus, green waste (water hyacinth) can be utilized to isolate bacterial species in anticipation of their application in heavy metal removal in liquid and solid waste sources for MICRO-BIOREMEDIATION.

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    APA: Copy

    VISHAN, ISHA, SIVAPRAKASAM, SENTHILKUMAR, & KALAMDHAD, AJAY. (2017). ISOLATION AND IDENTIFICATION OF BACTERIA FROM ROTARY DRUM COMPOST OF WATER HYACINTH. INTERNATIONAL JOURNAL OF RECYCLING OF ORGANIC WASTE IN AGRICULTURE, 6(3), 245-253. SID. https://sid.ir/paper/712233/en

    Vancouver: Copy

    VISHAN ISHA, SIVAPRAKASAM SENTHILKUMAR, KALAMDHAD AJAY. ISOLATION AND IDENTIFICATION OF BACTERIA FROM ROTARY DRUM COMPOST OF WATER HYACINTH. INTERNATIONAL JOURNAL OF RECYCLING OF ORGANIC WASTE IN AGRICULTURE[Internet]. 2017;6(3):245-253. Available from: https://sid.ir/paper/712233/en

    IEEE: Copy

    ISHA VISHAN, SENTHILKUMAR SIVAPRAKASAM, and AJAY KALAMDHAD, “ISOLATION AND IDENTIFICATION OF BACTERIA FROM ROTARY DRUM COMPOST OF WATER HYACINTH,” INTERNATIONAL JOURNAL OF RECYCLING OF ORGANIC WASTE IN AGRICULTURE, vol. 6, no. 3, pp. 245–253, 2017, [Online]. Available: https://sid.ir/paper/712233/en

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