EVALUATION OF THE CORRELATION BETWEEN THE PRESENCE OF SHIGA TOXINPRODUCING GENE (STX1 AND STX2) IN ESCHERICHIA COLI O157 H7 WITH BIOLOGICAL AND CHEMICAL PARAMETERS OF RAW MILK

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ANSARI S.; YAVARMANESH M.

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Journal: IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY Year:2017 | Volume:14 | Issue:70 Page(s): 283-291

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Title

EVALUATION OF THE CORRELATION BETWEEN THE PRESENCE OF SHIGA TOXINPRODUCING GENE (STX1 AND STX2) IN ESCHERICHIA COLI O157 H7 WITH BIOLOGICAL AND CHEMICAL PARAMETERS OF RAW MILK

Author(s)

ANSARI S. | YAVARMANESH M. | Issue Writer Certificate

Keywords

RAW MILKQ2

E. COLI O157 H7Q2

SHIGA TOXINQ1

MICROBIAL PARAMETERSQ1

CHEMICAL PARAMETERSQ1

Abstract

One of the most important SHIGA TOXIN-producer in dairy product is Escherichia coli O157. Milk pasteurization has not any effect on toxicity of the shigatoxin; it’s just preventing of microbial growth.Therefore, rapid and accurate detection of E.coli O157 can help to prevent food poisoning. The aim of this study was to evaluate the correlation between the presence of SHIGA TOXIN-producing genes (stx1 and stx2) with microbial and CHEMICAL PARAMETERS in RAW MILK.30 samples of RAW MILK randomly were collected from dairy product distributors of Mashhad. Isolation of E. COLI O157 H7 was done by specific culture. After DNA extraction, of 16s rRNA, stx1 and stx2 gene was screened by Polymerase Chain Reaction (PCR). The specific culture and PCR results showed that 62% and 89% of samples were contaminated by E. COLI O157 H7, respectively. Also, Stx1 gene was detected in 88% of SHIGA TOXIN E.coli and Stx2 gene was not present in any of the isolates tested in this study. The correlation between the presences of SHIGA TOXIN-producing gene (Stx1) with mesophilic bacteria enumeration, enumeration of E.coli, acidity, pH, Non-fat dry matter, fat content and domain of electro condactivity was estimated 0.174, 0.474, 0.992, 0.177, 0.724, 0.622 and 0.897, respectively. Our findings indicated that there is high correlation between CHEMICAL PARAMETERS of RAW MILK with the presence of shigatoxin genes in E. COLI O157 H7.

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APA: Copy

ANSARI, S., & YAVARMANESH, M.. (2017). EVALUATION OF THE CORRELATION BETWEEN THE PRESENCE OF SHIGA TOXINPRODUCING GENE (STX1 AND STX2) IN ESCHERICHIA COLI O157 H7 WITH BIOLOGICAL AND CHEMICAL PARAMETERS OF RAW MILK. IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 14(70), 283-291. SID. https://sid.ir/paper/71585/en

Vancouver: Copy

ANSARI S., YAVARMANESH M.. EVALUATION OF THE CORRELATION BETWEEN THE PRESENCE OF SHIGA TOXINPRODUCING GENE (STX1 AND STX2) IN ESCHERICHIA COLI O157 H7 WITH BIOLOGICAL AND CHEMICAL PARAMETERS OF RAW MILK. IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY[Internet]. 2017;14(70):283-291. Available from: https://sid.ir/paper/71585/en

IEEE: Copy

S. ANSARI, and M. YAVARMANESH, “EVALUATION OF THE CORRELATION BETWEEN THE PRESENCE OF SHIGA TOXINPRODUCING GENE (STX1 AND STX2) IN ESCHERICHIA COLI O157 H7 WITH BIOLOGICAL AND CHEMICAL PARAMETERS OF RAW MILK,” IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, vol. 14, no. 70, pp. 283–291, 2017, [Online]. Available: https://sid.ir/paper/71585/en

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