Comparing Efficacy of Ethidium Monoazide (EMA) and Propidium Monoazide (PMA) qPCR in Live Pathogen Quantifying by Real-Time PCR in Food Model Systems

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AZIZKHANI M.; TOORYAN F.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY Year:2019 | Volume:16 | Issue:90 Page(s): 377-386

Download Full-Text

Persian Verion

View:

327

Download:

Cites:

Information Journal Paper

Title

Comparing Efficacy of Ethidium Monoazide (EMA) and Propidium Monoazide (PMA) qPCR in Live Pathogen Quantifying by Real-Time PCR in Food Model Systems

Author(s)

AZIZKHANI M. | TOORYAN F. | Issue Writer Certificate

Keywords

Ethidium monoazideQ1

Food model systemQ1

PathogenQ1

PCRQ1

Propidium monoazideQ1

Abstract

PCR quantifies dead cells beside live cells and this makes the judgment of microbial quality of food samples complicated. The objective of this study was comparing the efficacy of Ethidium monoazideqPCR and Propidium monoazide-qPCR in quantifying live Pathogen cells (E. coli, Staphylococcus aureus, Enterococcus fecalis and Listeria monocyogenes) by real time PCR, in low-fat and high-fat milk, lactic cheese (low-fat) and processed cheese (high-fat). Different proportions of live and heat killed Pathogen cells were inoculated into food samples. After separating bacterial pellets, EMA and PMA treatments qPCR was conducted. One-way ANOVA followed by Turkey’ s Multiple Comparison tests were applied to analyze the data. In low-fat milk, EMA treatment resulted in 18, 20, 23 and 30% decrease in cell count of E. coli, S. aureus, E. fecalis and L. monocyogenes, respectively, compared to conventional culturing. Also, following treatment by PMA, 6, 3, 8 and 12% decrease in cell count was obtained for E. coli, S. aureus, E. fecalis and L. monocyogenes, respectively, compared to conventional culturing. In high-fat samples as processed cheese, a reduction of 20, 27, 30 and 25% in EMA treatment and 9, 6, 5 and 10% in PMA treatment was observed in cell count of E. coli, S. aureus, E. fecalis and L. monocyogenes, respectively. The inhibitory potential of EMA and PMA against signal emission was variable in different bacterial species and the fat content of the samples exerted no significant effect (p>0. 05) on PMA and EMA functionality.

Cites

No record.

References

No record.

Cite

APA: Copy

AZIZKHANI, M., & TOORYAN, F.. (2019). Comparing Efficacy of Ethidium Monoazide (EMA) and Propidium Monoazide (PMA) qPCR in Live Pathogen Quantifying by Real-Time PCR in Food Model Systems. IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 16(90 ), 377-386. SID. https://sid.ir/paper/71763/en

Vancouver: Copy

AZIZKHANI M., TOORYAN F.. Comparing Efficacy of Ethidium Monoazide (EMA) and Propidium Monoazide (PMA) qPCR in Live Pathogen Quantifying by Real-Time PCR in Food Model Systems. IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY[Internet]. 2019;16(90 ):377-386. Available from: https://sid.ir/paper/71763/en

IEEE: Copy

M. AZIZKHANI, and F. TOORYAN, “Comparing Efficacy of Ethidium Monoazide (EMA) and Propidium Monoazide (PMA) qPCR in Live Pathogen Quantifying by Real-Time PCR in Food Model Systems,” IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, vol. 16, no. 90 , pp. 377–386, 2019, [Online]. Available: https://sid.ir/paper/71763/en

Related Journal Papers