Gamma aminobutyric acid synthesis by Lactococcus lactis NZ1330 in dairy sludge medium with monosodium glutamate

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ALIZADEH BEHBAHANI B.; FALAH F.; Vasiee A.; TABATABAEI YAZDI F.; Mortazavi S. A.; Afsharian Sh.

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Journal: IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY Year:2020 | Volume:16 | Issue:97 Page(s): 89-100

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Title

Gamma aminobutyric acid synthesis by Lactococcus lactis NZ1330 in dairy sludge medium with monosodium glutamate

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Keywords

GABAQ1

Lactococcus lactisQ2

Dairy sludgeQ1

Monosodium glutamateQ1

Abstract

Gamma Aminobutyric Acid (GABA) is a bioactive molecule with different physiological roles in the body that inhibits neuronal stimulation and inhibits the delivery of stress-containing messages, has a calming effect and is used to treat diseases. Different has an effective role. In the present study, the possibility of producing this amino acid by Lactococcus lactis NZ1330 was investigated. In order to optimize the fermentation process three levels of Dairy sludge (5, 10 and 15%), Monosodium glutamate (0, 0. 5 and 1%) were selected at 24, 48 and 72 hours after fermentation. The presence of GABA in the culture medium was investigated by thin layer chromatography. Spectrophotometric method was used to quantify the bands present in thin-layer chromatography. Optimization results at 95% significance level showed that the optimum treatment consisted of medium containing 11. 2% Dairy sludge, 0. 7% Monosodium glutamate and 70 hours fermentation at 32 ° C and under these conditions, GABA production was ppm. It's 400. Therefore, this combination of media can be used as a suitable substrate for the production of valuable GABA drug and bioactive compounds.

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APA: Copy

ALIZADEH BEHBAHANI, B., FALAH, F., Vasiee, A., TABATABAEI YAZDI, F., Mortazavi, S. A., & Afsharian, Sh.. (2020). Gamma aminobutyric acid synthesis by Lactococcus lactis NZ1330 in dairy sludge medium with monosodium glutamate. IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 16(97 ), 89-100. SID. https://sid.ir/paper/71817/en

Vancouver: Copy

ALIZADEH BEHBAHANI B., FALAH F., Vasiee A., TABATABAEI YAZDI F., Mortazavi S. A., Afsharian Sh.. Gamma aminobutyric acid synthesis by Lactococcus lactis NZ1330 in dairy sludge medium with monosodium glutamate. IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY[Internet]. 2020;16(97 ):89-100. Available from: https://sid.ir/paper/71817/en

IEEE: Copy

B. ALIZADEH BEHBAHANI, F. FALAH, A. Vasiee, F. TABATABAEI YAZDI, S. A. Mortazavi, and Sh. Afsharian, “Gamma aminobutyric acid synthesis by Lactococcus lactis NZ1330 in dairy sludge medium with monosodium glutamate,” IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, vol. 16, no. 97 , pp. 89–100, 2020, [Online]. Available: https://sid.ir/paper/71817/en

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