DISCRIMINATION OF SELF COMPATIBLITY IN GENOTYPES OBTAINED FROM ALMOND CROSSING USING FLUORESCENT MICROSCOPY AND PCR METHODS

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EBADI A.; MOMENPOUR A.; IMANI A.

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Paper Information

Journal: IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY Year:2011 | Volume:12 | Issue:2 Page(s): 119-130

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Title

DISCRIMINATION OF SELF COMPATIBLITY IN GENOTYPES OBTAINED FROM ALMOND CROSSING USING FLUORESCENT MICROSCOPY AND PCR METHODS

Author(s)

EBADI A. | MOMENPOUR A. | IMANI A. | Issue Writer Certificate

Keywords

DOUBTFUL GENOTYPEQ2

SELF-COMPATIBLEQ3

SELF-INCOMPATIBLEQ2

SF ALLELEQ2

Abstract

Self-incompatibility is one of the most important constrains in almond production which reduce fruit set dramatically and makes orchard management difficult. Therefore, almond breeding to produce SELF-COMPATIBLE genotypes is very important. In this research, pollen tube growth was studied in 38 genotypes obtained from crossing between ‘Touno’ (male parent) and ‘Shahrood 12’ (female parent) by fluorescent microscopy and PCR. To study pollen tube growth after self pollination, flower samples were harvested at 120 h after hand pollination and pollen tube growth was studied by fluorescent microscopy. Results showed that at 120 h after hand pollination, 39.47% of genotypes were SELF-INCOMPATIBLE, 23.68% of genotypes were doubtful, 26.32% of genotypes were SELF-COMPATIBLE and 10.53% of genotypes were recognized as highly SELF-COMPATIBLE. To double check the status of selfcompatible genotype and to determinate the situation of DOUBTFUL GENOTYPEs recognized by fluorescent microscopy, PCR reaction was carried out by specific primers of SfF and SfR.Results showed that five genotype were SELF-COMPATIBLE. However, four another genotype were recognized as SELF-INCOMPATIBLE. In general, 50% of genotypes were realized as selfcompatible and another 50% were SELF-INCOMPATIBLE.

Cites

بررسی ابعاد اقتصادی تولید بادام کوهی در استان چهارمحال و بختیاری

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APA: Copy

EBADI, A., MOMENPOUR, A., & IMANI, A.. (2011). DISCRIMINATION OF SELF COMPATIBLITY IN GENOTYPES OBTAINED FROM ALMOND CROSSING USING FLUORESCENT MICROSCOPY AND PCR METHODS. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, 12(2), 119-130. SID. https://sid.ir/paper/80796/en

Vancouver: Copy

EBADI A., MOMENPOUR A., IMANI A.. DISCRIMINATION OF SELF COMPATIBLITY IN GENOTYPES OBTAINED FROM ALMOND CROSSING USING FLUORESCENT MICROSCOPY AND PCR METHODS. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY[Internet]. 2011;12(2):119-130. Available from: https://sid.ir/paper/80796/en

IEEE: Copy

A. EBADI, A. MOMENPOUR, and A. IMANI, “DISCRIMINATION OF SELF COMPATIBLITY IN GENOTYPES OBTAINED FROM ALMOND CROSSING USING FLUORESCENT MICROSCOPY AND PCR METHODS,” IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, vol. 12, no. 2, pp. 119–130, 2011, [Online]. Available: https://sid.ir/paper/80796/en

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