EVALUATION OF GENETIC DIVERSITY OF ‘GOLAB’ APPLE GENOTYPES USING MICROSATELLITE (SSR) MARKERS

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NAGHSHIN F.; BAHAR M.; TABATABAEI B.E.S.; HAJNAJARI H.

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Paper Information

Journal: IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY Year:2008 | Volume:9 | Issue:2 Page(s): 69-82

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Information Journal Paper

Title

EVALUATION OF GENETIC DIVERSITY OF ‘GOLAB’ APPLE GENOTYPES USING MICROSATELLITE (SSR) MARKERS

Author(s)

NAGHSHIN F. | BAHAR M. | TABATABAEI B.E.S. | HAJNAJARI H. | Issue Writer Certificate

Keywords

APPLESQ2

GENETIC DIVERSITYQ3

MALUS DOMESTICAQ3

MICROSATELLITEQ3

SSR MARKERQ3

Abstract

To investigate the genetic relatedness of Iranian local APPLES with introduced commercial cultivars and to evaluate the GENETIC DIVERSITY among local ‘Golab’ APPLES, 27 genotypes were assessed at genomic DNA level with 28 primer pairs of Simple Sequence Repeat (SSR). Twenty two SSR primers that produced distinct and scorable DNA bands were considered for analysis. The number of SSR primer loci detected among the 27 genotypes ranged from three to 11 alleles with an average of 5.4 alleles per primer. The polymorphic information content (PIC) among accessions varied from 0.37 to 0.77 with a mean of 0.68, showing high level of heterozygosis. The dendrogram constructed using the unweighted pair group method with arithmetic (UPGMA) method grouped the cultivars into five sub-clusters according to their geographical origins. Based on the dendrogram, clear-cut separation was found between Iranian APPLES and introduced cultivars. All the non- Iranian accessions jointly with one local apple, Ahar-2, were clustered together, while Iranian genotypes were classified in separate clusters. The closest genetic similarity was found among ‘Golab’ genotypes collected from different regions. The close relationship among Iranian ‘Golab’ APPLES as well as their similar morphological traits supports the hypothesis that Iranian ‘Golab’ APPLES possibly originated from a common ancestor.

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APA: Copy

NAGHSHIN, F., BAHAR, M., TABATABAEI, B.E.S., & HAJNAJARI, H.. (2008). EVALUATION OF GENETIC DIVERSITY OF ‘GOLAB’ APPLE GENOTYPES USING MICROSATELLITE (SSR) MARKERS. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, 9(2), 69-82. SID. https://sid.ir/paper/80887/en

Vancouver: Copy

NAGHSHIN F., BAHAR M., TABATABAEI B.E.S., HAJNAJARI H.. EVALUATION OF GENETIC DIVERSITY OF ‘GOLAB’ APPLE GENOTYPES USING MICROSATELLITE (SSR) MARKERS. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY[Internet]. 2008;9(2):69-82. Available from: https://sid.ir/paper/80887/en

IEEE: Copy

F. NAGHSHIN, M. BAHAR, B.E.S. TABATABAEI, and H. HAJNAJARI, “EVALUATION OF GENETIC DIVERSITY OF ‘GOLAB’ APPLE GENOTYPES USING MICROSATELLITE (SSR) MARKERS,” IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, vol. 9, no. 2, pp. 69–82, 2008, [Online]. Available: https://sid.ir/paper/80887/en

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