EFFECTS OF SOME VARIABLES ON SOMATIC EMBRYOGENESIS OF PETUNIA (PETUNIA HYBRIDA L.) AND ORNAMENTAL TOBACCO (NICOTIANA SPP.)

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SHEKAFANDEH A.; SEIBI M.

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Journal: IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY Year:2000 | Volume:1 | Issue:1-2 Page(s): 15-28

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Title

EFFECTS OF SOME VARIABLES ON SOMATIC EMBRYOGENESIS OF PETUNIA (PETUNIA HYBRIDA L.) AND ORNAMENTAL TOBACCO (NICOTIANA SPP.)

Author(s)

SHEKAFANDEH A. | SEIBI M. | Issue Writer Certificate

Keywords

ORNAMENTAL TOBACCOQ3

PETUNIAQ3

SOMATIC EMBRYOGENESISQ3

Abstract

Plant regeneration via SOMATIC EMBRYOGENESIS from different parts of flowers in some genotypes of PETUNIA and ORNAMENTAL TOBACCO was investigated. Also the effects of cold treatments, light, darkness and culture medium with different combinations of growth regulators were studied. After sterilization, explants were cultured aseptically. The results showed that the genotypes and different explants from the same genotype affected differently the callus and plant regeneration. Maximum regenerated plants from pedicel culture in PETUNIA (Pc 622 genotype) and in tobacco (N. glutinosa Linn. genotype) were 16% and 179%, respectively. The best culture medium was Murashage and Skoog (MS) with 3 mg 1-1 BAP and 0.2 mg 1-1 NAA. On this medium, the two mentioned genotypes regenerated 90 and 562 % plants from pedicel culture, respectively. No regeneration was obtained from different explants without cold treatment and a period of 7 to 9 days cold treatment increased the rate of regeneration in different genotypes of PETUNIA and tobacco. The effects of light and dark treatments on the rate of regeneration in N. glutinosa were depended to explants. In N. glutinosa regenerated plants from ovary was 10% in darkness and 2.4% in light, while for explants obtained from pedicel, adversely, there were 226% and 112.5% in light and dark, respectively.

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APA: Copy

SHEKAFANDEH, A., & SEIBI, M.. (2000). EFFECTS OF SOME VARIABLES ON SOMATIC EMBRYOGENESIS OF PETUNIA (PETUNIA HYBRIDA L.) AND ORNAMENTAL TOBACCO (NICOTIANA SPP.). IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, 1(1-2), 15-28. SID. https://sid.ir/paper/80902/en

Vancouver: Copy

SHEKAFANDEH A., SEIBI M.. EFFECTS OF SOME VARIABLES ON SOMATIC EMBRYOGENESIS OF PETUNIA (PETUNIA HYBRIDA L.) AND ORNAMENTAL TOBACCO (NICOTIANA SPP.). IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY[Internet]. 2000;1(1-2):15-28. Available from: https://sid.ir/paper/80902/en

IEEE: Copy

A. SHEKAFANDEH, and M. SEIBI, “EFFECTS OF SOME VARIABLES ON SOMATIC EMBRYOGENESIS OF PETUNIA (PETUNIA HYBRIDA L.) AND ORNAMENTAL TOBACCO (NICOTIANA SPP.),” IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, vol. 1, no. 1-2, pp. 15–28, 2000, [Online]. Available: https://sid.ir/paper/80902/en

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