IDENTIFICATION OF S-ALLELES IN SOME APRICOT CULTIVARS AND THEIR SEGREGATIONS IN SOME GENOTYPES USING SPECIFIC PRIMERS

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HAJILOU J.; MOHAMMADI S.A.; GERIGOURIAN V.; NAZEMIEH A.; BURGOS L.

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Journal: IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY Year:2006 | Volume:7 | Issue:2 Page(s): 103-114

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Title

IDENTIFICATION OF S-ALLELES IN SOME APRICOT CULTIVARS AND THEIR SEGREGATIONS IN SOME GENOTYPES USING SPECIFIC PRIMERS

Author(s)

Keywords

APRICOTQ4

S-ALLELEQ3

SPECIFIC PRIMERSQ3

PCRQ3

Abstract

To select suitable and compatible pollinizers and also to facilitate breeding programs in fruit trees, the genotype with S alleles in each cultivar should be determined. Because of the juvenile period of fruit trees, traditional methods are costly and time consuming. More recently, an easy and reliable identification of S-ALLELEs by PCR is of great interest. In order to identify the genotype of S-ALLELEs, genomic DNA from young leaves of nine APRICOT cultivars and also segregating progenies from a breeding program in Spain, was extracted. PCR amplification was carried out using SPECIFIC PRIMERS. Results showed that in all studied cultivars S-ALLELEs were amplified with SPECIFIC PRIMERS designed based on the first and second introns. Self-compatible cultivars shared the same band with 'Currot' as a Spanish self-compatible cultivar. Two out of the nine cultivars showed the same bands for S-ALLELEs. S-genotypes in progenies from 'Goldrich'×'Pepito' cross was detected as ScS1 and ScS2 in 6 and 3 seedlings, respectively. Furthermore, self-compatible progenies were determined when self-compatible cultivars were crossed together or with self-incompatible ones.

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APA: Copy

HAJILOU, J., MOHAMMADI, S.A., GERIGOURIAN, V., NAZEMIEH, A., & BURGOS, L.. (2006). IDENTIFICATION OF S-ALLELES IN SOME APRICOT CULTIVARS AND THEIR SEGREGATIONS IN SOME GENOTYPES USING SPECIFIC PRIMERS. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, 7(2), 103-114. SID. https://sid.ir/paper/80908/en

Vancouver: Copy

HAJILOU J., MOHAMMADI S.A., GERIGOURIAN V., NAZEMIEH A., BURGOS L.. IDENTIFICATION OF S-ALLELES IN SOME APRICOT CULTIVARS AND THEIR SEGREGATIONS IN SOME GENOTYPES USING SPECIFIC PRIMERS. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY[Internet]. 2006;7(2):103-114. Available from: https://sid.ir/paper/80908/en

IEEE: Copy

J. HAJILOU, S.A. MOHAMMADI, V. GERIGOURIAN, A. NAZEMIEH, and L. BURGOS, “IDENTIFICATION OF S-ALLELES IN SOME APRICOT CULTIVARS AND THEIR SEGREGATIONS IN SOME GENOTYPES USING SPECIFIC PRIMERS,” IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, vol. 7, no. 2, pp. 103–114, 2006, [Online]. Available: https://sid.ir/paper/80908/en

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