IDENTIFICATION AND DISPERSION OF BEMISIA TABACI (GENN.) AND BEMISIA ARGENTIFOLII BELLOWS AND PERRING IN COTTON FIELDS IN IRAN USING RAPD-PCR TECHNIQUE

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SAMI MOHAMMAD AMIN; KAMALI K.; JALALI JAVARAN MOKHTAR; TALEBI ALI ASGHAR

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Journal: IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE) Year:2006 | Volume:37 | Issue:3 Page(s): 413-424

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Title

IDENTIFICATION AND DISPERSION OF BEMISIA TABACI (GENN.) AND BEMISIA ARGENTIFOLII BELLOWS AND PERRING IN COTTON FIELDS IN IRAN USING RAPD-PCR TECHNIQUE

Author(s)

SAMI MOHAMMAD AMIN | KAMALI K. | JALALI JAVARAN MOKHTAR | TALEBI ALI ASGHAR | Issue Writer Certificate

Keywords

COTTON WHITEFLYQ2

GENETIC DIVERSITYQ3

BEMISIA TABACIQ3

BEMISIA ARGENTIFOLIIQ3

RAPD-PCRQ3

Abstract

The sweetpotato whitefly, BEMISIA TABACI (Genn.) and silverleaf whitefly B. argentifolii Bellows and Perring (Horn.: Aleyrodidae) are major pests of field crops, vegetables and ornamentals in Iran. Economic losses from this pest may either be caused through viral transmission, the excretion of honeydew or by direct damage to the plants. RAPD technique was employed to detect GENETIC DIVERSITY and dispersal of 11 BEMISIA TABACI populations during 2002. The infested leaves bearing nymphs and pupae were collected from Darab, Qom, Saveh, Gonbad, Gorgan, Varamin, Garmsar, Orzoiieh (Kerman), Rafsanjan, Shooshtar, and College of Agriculture (T.M.U.) cotton fields. Nymphs and pupae were cultured in a growth chamber under 24±2°C, 55±3% RH and 16:8 (L: D) photoperiod conditions. Five decamer primers were used in amplification reactions. All primers produced polymorphic bands. In general 71 RAPD markers were detected. Amplified DNA fragments ranged in size from 200 to 3000 bp. The results revealed that except for Rafsanjan, all other populations consisted of B. tabaci and B. argentifolii. The results indicated that RAPD marker is useful to the study of DNA polymorphism in B. tabaci.

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APA: Copy

SAMI, MOHAMMAD AMIN, KAMALI, K., JALALI JAVARAN, MOKHTAR, & TALEBI, ALI ASGHAR. (2006). IDENTIFICATION AND DISPERSION OF BEMISIA TABACI (GENN.) AND BEMISIA ARGENTIFOLII BELLOWS AND PERRING IN COTTON FIELDS IN IRAN USING RAPD-PCR TECHNIQUE. IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE), 37(3), 413-424. SID. https://sid.ir/paper/8332/en

Vancouver: Copy

SAMI MOHAMMAD AMIN, KAMALI K., JALALI JAVARAN MOKHTAR, TALEBI ALI ASGHAR. IDENTIFICATION AND DISPERSION OF BEMISIA TABACI (GENN.) AND BEMISIA ARGENTIFOLII BELLOWS AND PERRING IN COTTON FIELDS IN IRAN USING RAPD-PCR TECHNIQUE. IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE)[Internet]. 2006;37(3):413-424. Available from: https://sid.ir/paper/8332/en

IEEE: Copy

MOHAMMAD AMIN SAMI, K. KAMALI, MOKHTAR JALALI JAVARAN, and ALI ASGHAR TALEBI, “IDENTIFICATION AND DISPERSION OF BEMISIA TABACI (GENN.) AND BEMISIA ARGENTIFOLII BELLOWS AND PERRING IN COTTON FIELDS IN IRAN USING RAPD-PCR TECHNIQUE,” IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE), vol. 37, no. 3, pp. 413–424, 2006, [Online]. Available: https://sid.ir/paper/8332/en

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