AN IDENTIFICATION OF CLONAL LINEAGES IN POPULATIONS OF MAGNAPORTHE GRISEA USING REP-PCR AND A STUDY OF THEIR DISTRIBUTION IN GUILAN PROVINCE, IRAN

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

JAVAN NIKKHAH M.; HEJAROUD GH.A.; MCDONALD B.; SHARIFI TEHRANI A.; OKHOVAT S.M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE) Year:2007 | Volume:37 | Issue:6 Page(s): 1001-1015

Download Full-Text

Persian Verion

View:

896

Download:

Cites:

Information Journal Paper

Title

AN IDENTIFICATION OF CLONAL LINEAGES IN POPULATIONS OF MAGNAPORTHE GRISEA USING REP-PCR AND A STUDY OF THEIR DISTRIBUTION IN GUILAN PROVINCE, IRAN

Author(s)

Keywords

RICEQ2

BLASTQ3

MAGNAPOR THE GRISEAQ3

CLONAL LINEAGEQ3

HAPLOTYPEQ3

DISTRIBUTIONQ3

Abstract

One hundred and seventy monoconidial isolates of MAGNAPOR THE GRISEA, collected from different areas of Guilan province, were examined using rep-PCR genomic fingerprinting for charcterization of genetic diversity and for identification of CLONAL LINEAGEs in populations of the fungus. Isolates were recovered from ten common RICE cultivars in different RICE growing areas during 1997-1999. Two outwardly directed primer sequences designated from terminal inverted repeats of Pot2 (a repetitive transposable element specifically found throughout fungal genome) were used in this experiment. Fungal genomic DNA was extracted using phenol/ chloroform method for long PCR conditions, including increased extention time (10 min) and higher pH (9.2) used to amplify sequences lying between Pot2 elements. It generated variable length fragments ranging from 550 bp to longer than 5 kb. Each isolate was subjected to DNA fingerprinting, and both Pot2 lineage (isolates with ³70% band similarity), and HAPLOTYPE (isolates which had DNA fingerprints which differed by 5 to 30%) frequencies were determined. Phenetic analysis differentiated five distinct Pot 2 fingerprint lineages designated A, B, C, D and E. Among 170 isolates, 14, 2, 4, 3 and 18 HAPLOTYPEs were identified within lineages A, B, C, D, and E, respectively. Lineage E with frequency of appromixately 57.6% of all isolates made the most common and largest fingerprint group, recovered from all RICE cultivars sampled, especially local cultivar Tarom, and was distributed throughout Guilan. HAPLOTYPE F6, the most common HAPLOTYPE within lineage F, was recovered from almost all sampling sites. Lineage A, the second most common lineage, with 29% frequency, was found mainly in the central and western parts of Guilan. HAPLOTYPE AI, the most common HAPLOTYPE within lineage A, was found in most sampling sites in the western province. LineageA occurred at highest frequency on a susceptible local cultivar, Binam. Three other lineages totally exhibited appromixately 9.5% frequency. On the contrary to results of pathogenicity tests and number of pathotypes identified in the past, this investigation indicated a low level of genetic diversity in the M grisea population based on molecular method in Guilan. The result is similar to that reported in other countries. It is likely that the main reasons for a low level of diversity are a lack of sexual recombination, occurence of asexual reproduction and a short growing season for RICE in Guilan province. By asexual reproduction, clonal propagation occures in M grisea populations and is not affected by high variations.

Cites

No record.

References

Cite

APA: Copy

JAVAN NIKKHAH, M., HEJAROUD, GH.A., MCDONALD, B., SHARIFI TEHRANI, A., & OKHOVAT, S.M.. (2007). AN IDENTIFICATION OF CLONAL LINEAGES IN POPULATIONS OF MAGNAPORTHE GRISEA USING REP-PCR AND A STUDY OF THEIR DISTRIBUTION IN GUILAN PROVINCE, IRAN. IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE), 37(6), 1001-1015. SID. https://sid.ir/paper/8389/en

Vancouver: Copy

JAVAN NIKKHAH M., HEJAROUD GH.A., MCDONALD B., SHARIFI TEHRANI A., OKHOVAT S.M.. AN IDENTIFICATION OF CLONAL LINEAGES IN POPULATIONS OF MAGNAPORTHE GRISEA USING REP-PCR AND A STUDY OF THEIR DISTRIBUTION IN GUILAN PROVINCE, IRAN. IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE)[Internet]. 2007;37(6):1001-1015. Available from: https://sid.ir/paper/8389/en

IEEE: Copy

M. JAVAN NIKKHAH, GH.A. HEJAROUD, B. MCDONALD, A. SHARIFI TEHRANI, and S.M. OKHOVAT, “AN IDENTIFICATION OF CLONAL LINEAGES IN POPULATIONS OF MAGNAPORTHE GRISEA USING REP-PCR AND A STUDY OF THEIR DISTRIBUTION IN GUILAN PROVINCE, IRAN,” IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE), vol. 37, no. 6, pp. 1001–1015, 2007, [Online]. Available: https://sid.ir/paper/8389/en

Related Journal Papers