THE PRODUCTION OF HAPLOID EMBRYOS VIA MICROSPORE CULTURE OF WHEAT (TRITICUM AESTIVUM L.)

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GHARANJIK SH.; MOEINI AHMAD; MOUSAVI AMIR; ALIZADEH HOUSHANG

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Journal: IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE) Year:2007 | Volume:38-1 | Issue:2 (AGRONOMY AND CROP BIOTECHNOLOGY) Page(s): 267-275

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Title

THE PRODUCTION OF HAPLOID EMBRYOS VIA MICROSPORE CULTURE OF WHEAT (TRITICUM AESTIVUM L.)

Author(s)

GHARANJIK SH. | MOEINI AHMAD | MOUSAVI AMIR | ALIZADEH HOUSHANG | Issue Writer Certificate

Keywords

WHEATQ4

HAPLOIDQ3

MICROSPORE CULTUREQ2

EMBRYOGENESISQ3

TRITICUM AESTIVUMQ3

Abstract

In this study, eight commercial Iranian WHEAT cultivars were tested for their MICROSPORE CULTURE response. Spikes aseptically pretreated in strile inducer formulation [0.1g L-1 of 2-hydroxynicotinic acid, 10-6 mol L -1 2,4-D and 10-6 mol L -1 BAP] and placed in incubator at 33oC for a required period of time, ranging between about 48 and 72 h. Microspores were isolated through blending of spikes in microspore is olation soluting (0.3 mol L-1 mannitol). Isolated microspores were cocultured with immature ovaries in liquid NPB99 medium at a density of approximately 4x103 microspores mL-1 and incubated in the dark and at 27oC. After 3-4 weeks, response of WHEAT cultivars for EMBRYOGENESIS was evaluated. Cytological investigations using inverted microscope showed that multicellular structures, were formed in approximately one week after culture. During the second week, the microspore wall ruptured and immature embryoids emerged which grew into mature embryos within about 10 to 14 days. Analysis of variance indicated significant differences among cultivars for EMBRYOGENESIS that demonstrates the genetic dependence of this trait. Cultivars of Falat and Moghan1 with means of 1427±81.58 and 1108±54.55 embryos per spike respectively, were identified as the most superior cultivars in MICROSPORE CULTURE.

Cites

EFFECTS OF CULTIVAR, PRETREATMENT AND EMBRYO INDUCTION MEDIUM IN ISOLATED MICROSPORE CULTURE OF HEXAPLOID WHEAT (TRITICUM AESTIVUM L.)

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APA: Copy

GHARANJIK, SH., MOEINI, AHMAD, MOUSAVI, AMIR, & ALIZADEH, HOUSHANG. (2007). THE PRODUCTION OF HAPLOID EMBRYOS VIA MICROSPORE CULTURE OF WHEAT (TRITICUM AESTIVUM L.). IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE), 38-1(2 (AGRONOMY AND CROP BIOTECHNOLOGY)), 267-275. SID. https://sid.ir/paper/8457/en

Vancouver: Copy

GHARANJIK SH., MOEINI AHMAD, MOUSAVI AMIR, ALIZADEH HOUSHANG. THE PRODUCTION OF HAPLOID EMBRYOS VIA MICROSPORE CULTURE OF WHEAT (TRITICUM AESTIVUM L.). IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE)[Internet]. 2007;38-1(2 (AGRONOMY AND CROP BIOTECHNOLOGY)):267-275. Available from: https://sid.ir/paper/8457/en

IEEE: Copy

SH. GHARANJIK, AHMAD MOEINI, AMIR MOUSAVI, and HOUSHANG ALIZADEH, “THE PRODUCTION OF HAPLOID EMBRYOS VIA MICROSPORE CULTURE OF WHEAT (TRITICUM AESTIVUM L.),” IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE), vol. 38-1, no. 2 (AGRONOMY AND CROP BIOTECHNOLOGY), pp. 267–275, 2007, [Online]. Available: https://sid.ir/paper/8457/en

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