ASSESSMENT OF GENETIC DIVERSITY OF IRANIAN SWEET CHERRY (PRUNUS AVIUM L) GENOTYPES USING MICRO-SATELLITE MARKERS

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FARSAD A.; ESNA ASHARI M.; ERSHADI A.

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Journal: AGRICULTURAL RESEARCH Year:2008 | Volume:7 | Issue:4 (A) Page(s): 143-150

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Title

ASSESSMENT OF GENETIC DIVERSITY OF IRANIAN SWEET CHERRY (PRUNUS AVIUM L) GENOTYPES USING MICRO-SATELLITE MARKERS

Author(s)

FARSAD A. | ESNA ASHARI M. | ERSHADI A. | Issue Writer Certificate

Keywords

SWEET CHERRYQ3

MOLECULAR MARKERSQ3

GENETIC DIVERSITYQ2

MICRO-SATELLITEQ2

Abstract

Genetic diversity of 20 important Iranian SWEET CHERRY cultivars considerable for breeding programs, together with 3 sour-SWEET CHERRY, 1 sour cherry and 5 foreign SWEET CHERRY cultivars were studied using MICRO-SATELLITE markers. Extracted genomic DNA from SWEET CHERRY young leaves was used for PCR and amplification. Amplified products were separated using 6% polyacrylamide gel containing 7M urea under denaturing conditions and visualized by staining with silver nitrate. Gels were then scored based on either the presence or absence of the bands, -and polymorphism information content (PIC) for each SSR marker was determined. The number of alleles per locus ranged from 6 to 9 and totally 123 alleles were identified. GENETIC DIVERSITY of cherry cultivars was estimated using NTSYS software program based on the Jaccard Similarity coefficient which was considered for cluster analysis. Cluster analysis placed the 29 cultivars in five groups, showing a high diversity between the cultivars, so that the Iranian sweet cherries were alighted quite different from the foreign cherries. Foreign cherries were grouped in different branches so other sour cherry and sour-sweet cherries.

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APA: Copy

FARSAD, A., ESNA ASHARI, M., & ERSHADI, A.. (2008). ASSESSMENT OF GENETIC DIVERSITY OF IRANIAN SWEET CHERRY (PRUNUS AVIUM L) GENOTYPES USING MICRO-SATELLITE MARKERS. AGRICULTURAL RESEARCH, 7(4 (A)), 143-150. SID. https://sid.ir/paper/84682/en

Vancouver: Copy

FARSAD A., ESNA ASHARI M., ERSHADI A.. ASSESSMENT OF GENETIC DIVERSITY OF IRANIAN SWEET CHERRY (PRUNUS AVIUM L) GENOTYPES USING MICRO-SATELLITE MARKERS. AGRICULTURAL RESEARCH[Internet]. 2008;7(4 (A)):143-150. Available from: https://sid.ir/paper/84682/en

IEEE: Copy

A. FARSAD, M. ESNA ASHARI, and A. ERSHADI, “ASSESSMENT OF GENETIC DIVERSITY OF IRANIAN SWEET CHERRY (PRUNUS AVIUM L) GENOTYPES USING MICRO-SATELLITE MARKERS,” AGRICULTURAL RESEARCH, vol. 7, no. 4 (A), pp. 143–150, 2008, [Online]. Available: https://sid.ir/paper/84682/en

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