GHRELIN ENHANCES VIABILITY OF RAT SPERMATOZOA DURING INCUBATION AT 37°C

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

KHERADMAND A.; TAATI M.; BABAEI H.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR) Year:2009 | Volume:10 | Issue:2 (27) Page(s): 103-109

Download Full-Text

Persian Verion

View:

722

Download:

178

Cites:

Information Journal Paper

Title

GHRELIN ENHANCES VIABILITY OF RAT SPERMATOZOA DURING INCUBATION AT 37°C

Author(s)

KHERADMAND A. | TAATI M. | BABAEI H. | Issue Writer Certificate

Keywords

GHRELINQ2

SPERMATOZOAQ3

ANTIOXIDANT PROPERTIESQ1

RATQ2

HOS TESTQ3

Abstract

Antioxidant properties of GHRELIN have been demonstrated in recent studies. In the present study, the effects of chronic administration of GHRELIN on the motility and plasma membrane integrity of RAT SPERMATOZOA during incubation at 37°C were investigated. Thirty 45-day-old male Wistar RATs were divided into control and treatment groups. RATs in the treatment group were daily injected subcutaneously with 1 nmol of GHRELIN for 10 consecutive days and the control RATs received normal saline. Sperm was collected after killing of RATs on days 5, 15 and 40 after the last injection, and sperm characteristics were examined at 0, 3 and 5 h after incubation at 37°C. Mass motility and forward progressive movement of SPERMATOZOA were significantly higher in GHRELIN-treated animals at 3 and 5 h of incubation on day 5 (P<0.05). After 3 h of incubation on day 15, only mass motility was greater than that of the control group. Plasma membrane integrity was assessed by hypoosmotic swelling (HOS) “water test”. The mean value of HOS reacted SPERMATOZOA was higher in the treatment group on days 5 and 15 during 0, 3 and 5 h of incubation (P<0.05). However, the percentage of HOS-positive SPERMATOZOA was not significantly different on day 40 between groups. There was a high correlation at 3 and 5 h of day 5 between the forward progressive movement (r = 0.92 and 0.94, P<0.0001) as well as overall sperm motility (r = 0.78 and 0.81, P<0.01) with HOS TEST in the GHRELIN-treated animals. These results can be attributed to the antioxidative effects of GHRELIN on the RAT sperm especially on its plasma membrane which probably protects the sperm plasma membrane against oxidative damage during incubation and causes subsequent significant increase in the HOS TEST results. This may result in higher sperm motility index during 5 h of incubation.

Cites

No record.

References

No record.

Cite

APA: Copy

KHERADMAND, A., TAATI, M., & BABAEI, H.. (2009). GHRELIN ENHANCES VIABILITY OF RAT SPERMATOZOA DURING INCUBATION AT 37°C. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), 10(2 (27)), 103-109. SID. https://sid.ir/paper/85726/en

Vancouver: Copy

KHERADMAND A., TAATI M., BABAEI H.. GHRELIN ENHANCES VIABILITY OF RAT SPERMATOZOA DURING INCUBATION AT 37°C. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR)[Internet]. 2009;10(2 (27)):103-109. Available from: https://sid.ir/paper/85726/en

IEEE: Copy

A. KHERADMAND, M. TAATI, and H. BABAEI, “GHRELIN ENHANCES VIABILITY OF RAT SPERMATOZOA DURING INCUBATION AT 37°C,” IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), vol. 10, no. 2 (27), pp. 103–109, 2009, [Online]. Available: https://sid.ir/paper/85726/en

Related Journal Papers