ESTIMATING MICROSATELLITE BASED GENETIC DIVERSITY IN RHODE ISLAND RED CHICKEN

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

DAS A.K.; KUMAR S.; RAHIM A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR) Year:2015 | Volume:16 | Issue:3 (52) Page(s): 274-277

Download Full-Text

Persian Verion

View:

810

Download:

153

Cites:

Information Journal Paper

Title

ESTIMATING MICROSATELLITE BASED GENETIC DIVERSITY IN RHODE ISLAND RED CHICKEN

Author(s)

DAS A.K. | KUMAR S. | RAHIM A. | Issue Writer Certificate

Keywords

F-STATISTICSQ2

HETEROZYGOSITYQ2

MICROSATELLITE ALLELEQ2

POLYMORPHIC INFORMATION CONTENTQ2

RHODE ISLAND RED CHICKENQ2

Abstract

This study aimed to estimate microsatellite based genetic diversity in two lines (the selected RIRS and control line RIRC) of Rhode Island Red (RIR) chicken. Genomic DNA of 24 randomly selected birds maintained at Central Avian Research Institute (India) and 24 microsatellite markers were used. MICROSATELLITE ALLELEs were determined on 6% urea-PAGE, recorded using GelDoc system and the samples were genotyped. Nei’s HETEROZYGOSITY and Botstein’s POLYMORPHIC INFORMATION CONTENT (PIC) at each microsatellite locus were estimated. Wright’s fixation indices and gene flow were estimated using POPGENE software. All the microsatellite loci were polymorphic and the estimated PIC ranged from 0.3648 (MCW0059) to 0.7819 (ADL0267) in RIRS and from 0.2392 (MCW0059) to 0.8620 (ADL0136) in RIRC. Most of the loci were highly informative (PIC>0.50) in the both lines, except for five loci in RIRS and six loci in RIRC line. Nei’s HETEROZYGOSITY per locus ranged from 0.4800 (MCW0059) to 0.8056 (ADL0267) in RIRS and from 0.2778 (MCW0059) to 0.875 (ADL0136) in RIRC. Out of 24 loci, 15 (62.5%) in RIRS and 14 loci (58.33%) in RIRC revealed moderate to high negative FIS index indicating heterozygote excess for these loci in corresponding lines, but the rest revealed positive FIS indicating HETEROZYGOSITY deficiency. A mean FIS across the both lines indicated overall 10.77% HETEROZYGOSITY deficit and a mean FIT indicated 17.19% inbreeding co-efficient favoring homozygosity over the two lines. The mean FST indicated that 10.18% of the microsatellite variation between the two lines was due to their genetic difference.

Cites

No record.

References

No record.

Cite

APA: Copy

DAS, A.K., KUMAR, S., & RAHIM, A.. (2015). ESTIMATING MICROSATELLITE BASED GENETIC DIVERSITY IN RHODE ISLAND RED CHICKEN. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), 16(3 (52)), 274-277. SID. https://sid.ir/paper/85751/en

Vancouver: Copy

DAS A.K., KUMAR S., RAHIM A.. ESTIMATING MICROSATELLITE BASED GENETIC DIVERSITY IN RHODE ISLAND RED CHICKEN. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR)[Internet]. 2015;16(3 (52)):274-277. Available from: https://sid.ir/paper/85751/en

IEEE: Copy

A.K. DAS, S. KUMAR, and A. RAHIM, “ESTIMATING MICROSATELLITE BASED GENETIC DIVERSITY IN RHODE ISLAND RED CHICKEN,” IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), vol. 16, no. 3 (52), pp. 274–277, 2015, [Online]. Available: https://sid.ir/paper/85751/en

Related Journal Papers