MOLECULAR PHYLOGENY OF SOME AVIAN SPECIES USING CYTOCHROME B GENE SEQUENCE ANALYSIS

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AWAD A.; KHALIL S.R.; ABD ELHAKIM Y.M.

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Paper Information

Journal: IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR) Year:2015 | Volume:16 | Issue:2 (51) Page(s): 218-222

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Information Journal Paper

Title

MOLECULAR PHYLOGENY OF SOME AVIAN SPECIES USING CYTOCHROME B GENE SEQUENCE ANALYSIS

Author(s)

AWAD A. | KHALIL S.R. | ABD ELHAKIM Y.M. | Issue Writer Certificate

Keywords

AVIAN SPECIESQ2

CYTOCHROME B GENEQ2

PHYLOGENETIC ANALYSISQ2

Abstract

Veritable identification and differentiation of AVIAN SPECIES is a vital step in conservative, taxonomic, forensic, legal and other ornithological interventions. Therefore, this study involved the application of molecular approach to identify some AVIAN SPECIES i.e. Chicken (Gallus gallus), Muskovy duck (Cairina moschata), Japanese quail (Coturnix japonica), Laughing dove (Streptopelia senegalensis), and Rock pigeon (Columba livia). Genomic DNA was extracted from blood samples and partial sequence of the mitochondrial CYTOCHROME B GENE (358 bp) was amplified and sequenced using universal primers. Sequences alignment and phylogenetic analyses were performed by CLC main workbench program. The obtained five sequences were deposited in GenBank and compared with those previously registered in GenBank. The similarity percentage was 88.60% between Gallus gallus and Coturnix japonicaand 80.46% between Gallus gallus and Columba livia. The percentage of identity between the studied species and GenBank species ranged from 77.20% (Columba oenas and Anas platyrhynchos) to 100% (Gallus gallus and Gallus sonneratii, Coturnix coturnixand Coturnix japonica, Meleagris gallopavo and Columba livia). Amplification of the partial sequence of mitochondrial CYTOCHROME B GENE proved to be practical for identification of an AVIAN SPECIES unambiguously.

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APA: Copy

AWAD, A., KHALIL, S.R., & ABD ELHAKIM, Y.M.. (2015). MOLECULAR PHYLOGENY OF SOME AVIAN SPECIES USING CYTOCHROME B GENE SEQUENCE ANALYSIS. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), 16(2 (51)), 218-222. SID. https://sid.ir/paper/85777/en

Vancouver: Copy

AWAD A., KHALIL S.R., ABD ELHAKIM Y.M.. MOLECULAR PHYLOGENY OF SOME AVIAN SPECIES USING CYTOCHROME B GENE SEQUENCE ANALYSIS. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR)[Internet]. 2015;16(2 (51)):218-222. Available from: https://sid.ir/paper/85777/en

IEEE: Copy

A. AWAD, S.R. KHALIL, and Y.M. ABD ELHAKIM, “MOLECULAR PHYLOGENY OF SOME AVIAN SPECIES USING CYTOCHROME B GENE SEQUENCE ANALYSIS,” IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), vol. 16, no. 2 (51), pp. 218–222, 2015, [Online]. Available: https://sid.ir/paper/85777/en

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