DETECTION AND DIFFERENTIATION OF WILD-TYPE AND VACCINE STRAINS OF CANINE DISTEMPER VIRUS BY A DUPLEX REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION

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DONG X.Y.; LI W.H.; ZHU J.L.; LIU W.J.; ZHAO M.Q.; LUO Y.W.; CHEN J.D.

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Paper Information

Journal: IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR) Year:2015 | Volume:16 | Issue:2 (51) Page(s): 172-175

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Title

DETECTION AND DIFFERENTIATION OF WILD-TYPE AND VACCINE STRAINS OF CANINE DISTEMPER VIRUS BY A DUPLEX REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION

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Keywords

CANINE DISTEMPER VIRUSQ2

DIFFERENTIATIONQ2

DUPLEX RT-PCRQ2

SENSITIVITYQ2

SPECIFICITYQ2

Abstract

CANINE DISTEMPER VIRUS (CDV) is the cause of canine distemper (CD) which is a severe and highly contagious disease in dogs. In the present study, a duplex reverse transcription polymerase chain reaction (RT-PCR) method was developed for the detection and DIFFERENTIATION of wild-type and vaccine strains of CDV. Four primers were designed to detect and discriminate the two viruses by generating 638- and 781-bp cDNA products, respectively. Furthermore, the DUPLEX RT-PCR method was used to detect 67 field samples suspected of CD from Guangdong province in China. Results showed that, 33 samples were to be wild-type-like. The DUPLEX RT-PCR method exhibited high SPECIFICITY and SENSITIVITY which could be used to effectively detect and differentiate wild-type and vaccine CDV, indicating its use for clinical detection and epidemiological surveillance.

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APA: Copy

DONG, X.Y., LI, W.H., ZHU, J.L., LIU, W.J., ZHAO, M.Q., LUO, Y.W., & CHEN, J.D.. (2015). DETECTION AND DIFFERENTIATION OF WILD-TYPE AND VACCINE STRAINS OF CANINE DISTEMPER VIRUS BY A DUPLEX REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), 16(2 (51)), 172-175. SID. https://sid.ir/paper/85778/en

Vancouver: Copy

DONG X.Y., LI W.H., ZHU J.L., LIU W.J., ZHAO M.Q., LUO Y.W., CHEN J.D.. DETECTION AND DIFFERENTIATION OF WILD-TYPE AND VACCINE STRAINS OF CANINE DISTEMPER VIRUS BY A DUPLEX REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR)[Internet]. 2015;16(2 (51)):172-175. Available from: https://sid.ir/paper/85778/en

IEEE: Copy

X.Y. DONG, W.H. LI, J.L. ZHU, W.J. LIU, M.Q. ZHAO, Y.W. LUO, and J.D. CHEN, “DETECTION AND DIFFERENTIATION OF WILD-TYPE AND VACCINE STRAINS OF CANINE DISTEMPER VIRUS BY A DUPLEX REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION,” IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), vol. 16, no. 2 (51), pp. 172–175, 2015, [Online]. Available: https://sid.ir/paper/85778/en

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