PITUITARY PRIMARY CELL CULTURE OF COMMON CARP (CYPRINUS CARPIO) AND EVALUATION OF ITS SECRETION EFFECT ON ENDOCRINE ACTIVITY OF INCUBATED OVARIAN FOLLICLES

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SALAMAT NEGIN; ALBOUGHOBISH N.; HASHEMI TABAR MAHMOUD; MESBAH M.; AHANGARPOUR A.

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Journal: IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR) Year:2009 | Volume:10 | Issue:1 (26) Page(s): 61-65

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Title

PITUITARY PRIMARY CELL CULTURE OF COMMON CARP (CYPRINUS CARPIO) AND EVALUATION OF ITS SECRETION EFFECT ON ENDOCRINE ACTIVITY OF INCUBATED OVARIAN FOLLICLES

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Keywords

PITUITARYQ2

CELL CULTUREQ2

STEROIDSQ3

RADIO IMMUNOASSAYQ3

COMMON CARPQ2

Abstract

In this study, five carp PITUITARY glands were collected and dispersed enzymatic ally and mechanically. Then, the cells were cultivated as monolayer in MEM (minimum essential medium Eagle). The culture media were collected after 72 h and frozen at -20oC. Carp ovarian follicles also were separated mechanically and incubated in BSS (basic salt solution) Cortland medium in 24-well micro plates for 48 h at 20oC. Then, they were divided into two groups: control group which were incubated in BSS medium and experimental group which subdivided into three subgroups according to treatment with different concentration of collected PITUITARY secretion (50, 100 and 200 ml/ml). Follicles culture media were collected 24 h later and were analyzed for 17-b-oestradiol (E2) and 17-a-hydroxy progesterone (P4) content by radioimmunoassay (RIA). The results showed that adding low concentration (50 ml/ml) of collected PITUITARY secretion (CPS) increased steroid hormones (E2 and P4) secretion of incubated ovarian follicles significantly (P<0.05) but the high concentration of CPS (200 ml/ml) significantly decreased the secretion of E2 and P4 (P<0.05). Collected PITUITARY secretion at the concentration of 100 ml/ml had no significant effect on steroid hormones (P>0.05).

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APA: Copy

SALAMAT, NEGIN, ALBOUGHOBISH, N., HASHEMI TABAR, MAHMOUD, MESBAH, M., & AHANGARPOUR, A.. (2009). PITUITARY PRIMARY CELL CULTURE OF COMMON CARP (CYPRINUS CARPIO) AND EVALUATION OF ITS SECRETION EFFECT ON ENDOCRINE ACTIVITY OF INCUBATED OVARIAN FOLLICLES. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), 10(1 (26)), 61-65. SID. https://sid.ir/paper/85803/en

Vancouver: Copy

SALAMAT NEGIN, ALBOUGHOBISH N., HASHEMI TABAR MAHMOUD, MESBAH M., AHANGARPOUR A.. PITUITARY PRIMARY CELL CULTURE OF COMMON CARP (CYPRINUS CARPIO) AND EVALUATION OF ITS SECRETION EFFECT ON ENDOCRINE ACTIVITY OF INCUBATED OVARIAN FOLLICLES. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR)[Internet]. 2009;10(1 (26)):61-65. Available from: https://sid.ir/paper/85803/en

IEEE: Copy

NEGIN SALAMAT, N. ALBOUGHOBISH, MAHMOUD HASHEMI TABAR, M. MESBAH, and A. AHANGARPOUR, “PITUITARY PRIMARY CELL CULTURE OF COMMON CARP (CYPRINUS CARPIO) AND EVALUATION OF ITS SECRETION EFFECT ON ENDOCRINE ACTIVITY OF INCUBATED OVARIAN FOLLICLES,” IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), vol. 10, no. 1 (26), pp. 61–65, 2009, [Online]. Available: https://sid.ir/paper/85803/en

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