POPULUS CASPICA INTERSPECIFIC HYBRID PRODUCTION USING OVARY CULTURE

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JAFARI MOFIDABADI A.; ZARIN BALL E.; ATEMAD V.; SHRIATE NEJAD SH.

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Journal: Journal of Agricultural Sciences and Natural Resources Year:2007 | Volume:13 | Issue:6 Page(s): 31-37

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Title

POPULUS CASPICA INTERSPECIFIC HYBRID PRODUCTION USING OVARY CULTURE

Author(s)

JAFARI MOFIDABADI A. | ZARIN BALL E. | ATEMAD V. | SHRIATE NEJAD SH. | Issue Writer Certificate

Keywords

OVARYQ3

EMBRYO CULTUREQ3

INTERSPECIFIC HYBRIDIZATIONQ3

POPULUS CASPICA AND P. ALBAQ3

Abstract

OVARY culture was used to develop interspecific hybrid plants between POPULUS CASPICA AND P. ALBA. Developed ovaris were isolated from female branches of Populus caspica, which were pollinated with P. Alba pollen grains using twig and pod breeding techniques. The same artificial pollination procedures have been done on female branches of Populus Alba with P.caspica pollen grains for reciprocal cross. Isolated ovaries were then transferred to growth regulator free half concentrated MS agar medium for embryo germination in Populus caspica x Palba cross. There were no significant differences between MS and Half-MS media for embryo development and germination.Highest embryo germination was observed in 14 days old embryos in (Populus caspica x Palba) and (Populus Alba x Pcaspica) crosses and showed significant differences at 0.05 level (23.9 and 53.12% respectively). Planlets reached 5 or 8 cm height were then cultured in the same medium within jars, before being transferred to the potting soil. Two hundred seventy four plantlets from both crosses were successfully acclimatized in green-house and transplanted in the field.

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APA: Copy

JAFARI MOFIDABADI, A., ZARIN BALL, E., ATEMAD, V., & SHRIATE NEJAD, SH.. (2007). POPULUS CASPICA INTERSPECIFIC HYBRID PRODUCTION USING OVARY CULTURE. JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, 13(6), 31-37. SID. https://sid.ir/paper/9177/en

Vancouver: Copy

JAFARI MOFIDABADI A., ZARIN BALL E., ATEMAD V., SHRIATE NEJAD SH.. POPULUS CASPICA INTERSPECIFIC HYBRID PRODUCTION USING OVARY CULTURE. JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES[Internet]. 2007;13(6):31-37. Available from: https://sid.ir/paper/9177/en

IEEE: Copy

A. JAFARI MOFIDABADI, E. ZARIN BALL, V. ATEMAD, and SH. SHRIATE NEJAD, “POPULUS CASPICA INTERSPECIFIC HYBRID PRODUCTION USING OVARY CULTURE,” JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, vol. 13, no. 6, pp. 31–37, 2007, [Online]. Available: https://sid.ir/paper/9177/en

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