Design of a biosensor using antibodies immobilized on the quantum dots and rhodamine attached to citrinin to detect mycotoxin

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Shojaee Sadi Behrooz; BAYAT MANSOUR; TAJIK PARVIZ; HASHEMI SEYED JAMAL

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN Year:2021 | Volume:17 | Issue:4 (71) Page(s): 3363-3371

Download Full-Text

Persian Verion

View:

250

Download:

Cites:

Information Journal Paper

Title

Design of a biosensor using antibodies immobilized on the quantum dots and rhodamine attached to citrinin to detect mycotoxin

Author(s)

Shojaee Sadi Behrooz | BAYAT MANSOUR | TAJIK PARVIZ | HASHEMI SEYED JAMAL | Issue Writer Certificate

Keywords

Nano-biosensor

rhodamine

citrinin

Quantum dot

Abstract

citrinine is a nephrotoxic metabolite that damages the kidneys in animals. Therefore, the development of detoxification methods during the food process is important. Quantum devices, using a single source of excitation, are excited by individual wavelengths and produce different emission peaks. In this study, a new bioconjugation-based sensor consisting of Quantum dot semiconductor nanocrystals and anti-citrinin antibody was used to determine the amount of citrinin toxins using the FRET (Fluorescence Resonance Energy Transfer) system. Semiconductor nanocrystals or quantum materials (CdTe, CdTe / CdS) were synthesized in the laboratory using reflux method by reducing tellurium powder under a stream of nitrogen gas. The fluorescence intensity of quantum data was determined using a spectrofluorimetric device. Antibody activity in nanobioconjugate was also measured by spectrofluorimetry. The citrinin-albumin conjugate was then attached to the fluorescence compound rhodamine 123, which is a highly affinity immunological reaction between the anti-citrinin-quantum antibody (donor) and the citrinin-rhodamine 123 (receptor) label used as fluorophora. After adding the nanobioconjugated solution to the cuvette, which contains a certain amount of phosphate buffer, citrinin was added to it in different concentrations, and after a few minutes, changes in the intensity of Quantum dot fluorescence were observed. A regular decrease in fluorescence intensity was obtained by increasing the citrinin concentration. Due to the collective effects of mycotoxins on human and animal health, it is better to determine the extent of food contamination with mycotoxins, including citrinin, using nanobiosensors as a specific method that results from the use of monoclonal antibodies. Unlike conventional methods such as ELISA and HPLC, these designed nanobiosensors are homogeneous, simple, fast, and inexpensive and do not require much separation or rinsing.

Cites

No record.

References

No record.

Cite

APA: Copy

Shojaee Sadi, Behrooz, BAYAT, MANSOUR, TAJIK, PARVIZ, & HASHEMI, SEYED JAMAL. (2021). Design of a biosensor using antibodies immobilized on the quantum dots and rhodamine attached to citrinin to detect mycotoxin. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, 17(4 (71) ), 3363-3371. SID. https://sid.ir/paper/951110/en

Vancouver: Copy

Shojaee Sadi Behrooz, BAYAT MANSOUR, TAJIK PARVIZ, HASHEMI SEYED JAMAL. Design of a biosensor using antibodies immobilized on the quantum dots and rhodamine attached to citrinin to detect mycotoxin. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN[Internet]. 2021;17(4 (71) ):3363-3371. Available from: https://sid.ir/paper/951110/en

IEEE: Copy

Behrooz Shojaee Sadi, MANSOUR BAYAT, PARVIZ TAJIK, and SEYED JAMAL HASHEMI, “Design of a biosensor using antibodies immobilized on the quantum dots and rhodamine attached to citrinin to detect mycotoxin,” JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, vol. 17, no. 4 (71) , pp. 3363–3371, 2021, [Online]. Available: https://sid.ir/paper/951110/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops

No record.