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Information Journal Paper

Title

The comparison of polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) methods for detecting Fasciola hepatica in faecal samples of sheeps in Lorestan province

Pages

  3305-3312

Abstract

 Fascioliasis is a zoonotic disease with long history in Iran. Different methods are used for detection of the disease and one of methods is LAMP. The present study was conducted to compare the LAMP and PCR methods for detection of Fasciola hepatica in fecal samples of sheeps in Lorestan province. During 3 months, 195 samples were collected by classical randomized method. The samples were stored in-20º C for future investigations by LAMP and PCR. The samples were investigated by LAMP and PCR. The data were analyzed by SPSS software and Chi-square and Kappa coefficient was used. The results showed that LAMP technique showed significant difference with LAMP and detected infected samples (11 samples in LAMP and 7 samples in PCR). The results for kappa showed a strong agreement between the both methods with coefficient of 0. 65 and P=0. 001). The LAMP method showed higher Sensitivity. In sum, LAMP method showed higher Sensitivity compared to other methods and can be used for detection of F. hepatica.

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    APA: Copy

    Amiri, Siyamak, SHEMSHADI, BAHAR, FALLAHI, SHIRZAD, & Shirali, Saloomeh. (2020). The comparison of polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) methods for detecting Fasciola hepatica in faecal samples of sheeps in Lorestan province. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, 17(3 (70) ), 3305-3312. SID. https://sid.ir/paper/953358/en

    Vancouver: Copy

    Amiri Siyamak, SHEMSHADI BAHAR, FALLAHI SHIRZAD, Shirali Saloomeh. The comparison of polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) methods for detecting Fasciola hepatica in faecal samples of sheeps in Lorestan province. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN[Internet]. 2020;17(3 (70) ):3305-3312. Available from: https://sid.ir/paper/953358/en

    IEEE: Copy

    Siyamak Amiri, BAHAR SHEMSHADI, SHIRZAD FALLAHI, and Saloomeh Shirali, “The comparison of polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) methods for detecting Fasciola hepatica in faecal samples of sheeps in Lorestan province,” JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, vol. 17, no. 3 (70) , pp. 3305–3312, 2020, [Online]. Available: https://sid.ir/paper/953358/en

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