Comparison of the effect of sodium bicarbonate buffer with Megasphaera elsdenii as a rumen-consuming acid on growth performance, digestibility, rumen, and blood parameters of lambs in high concentrate

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Khorasani Omid; CHAJI MORTEZA; BAGHBAN FARSHAD

Title

Comparison of the effect of sodium bicarbonate buffer with Megasphaera elsdenii as a rumen-consuming acid on growth performance, digestibility, rumen, and blood parameters of lambs in high concentrate

Author(s)

Khorasani Omid | CHAJI MORTEZA | BAGHBAN FARSHAD | Issue Writer Certificate

Keywords

Buffer

Lambs

Megasphaera elsdenii

Ruminal acidosis

Saccharomyces cerevisiae

Abstract

Introduction: Subacute acidosis is characterized by pH of 5. 5 and below, reduced feed intake and livestock performance, and leads to significant economic losses. Therefore, preventive measures to prevent the occurrence of acidosis and improved digestion of starch such as probiotics and Buffers have been considered. The feeding of high fermentable carbohydrates in ruminants resulted in producing high amounts of organic acids in the rumen, followed by a decrease in rumen pH. In the animals not adapted to high levels of carbohydrate fermentation, rumen lactate concentrations increase unacceptably, because populations of lactate-utilizing bacteria such as Selenomonas ruminantium and Megasphaera elsdenii are low and cannot rapidly match and their proliferation need more time (Chaucheyras-Durand et al. 2008). Reports show that the consumption of live yeast cells of Saccharomyces cerevisiae leads to the removal and consumption of oxygen in the rumen environment as well as the release of some essential enzymes, vitamins and other nutrients. These factors can significantly contribute to the proper life and activity of microorganisms in the ruminal environment. It has been suggested that the yeast of Saccharomyces cerevisiae may develop the population of Megasphaera elsdenii and increase lactate use (Calsamiglia et al. 2012). Megasphaera elsdenii prevents the sharp decrease in ruminal pH by consuming lactic acid as a result of lactic acid accumulation (Perbo et al. 2012). Due to the diverse ability of different species of Megasphaera elsdenii to produce volatile fatty acids under acidosis, further research can help find new species with high ability to use lactate (Sedighi and Alipour 2019). The use of yeast and bioactive compounds compared with the chemicals can be effective in reducing inflammation caused by acidosis (Aschenbach et al 2019). Material and methods: Twenty-four Arabic male Lambs with four months old and initial body weight of 23. 9± 3. 15 kg were used in a completely randomized design with three treatments and eight replicates. The trial period consisted of 77 days (11 weeks) including 14 days of habituation period and 63 days (9 weeks) of recording period. The Lambs were randomly assigned to one of the three treatments: 1-control (no additive) 2-control + Sodium bicarbonate (1% daily diet in two meals) 3control + Megasphaera elsdenii and Saccharomyces cerevisiae (bacterial-yeast). The amount of Megasphaera elsdenii was 3 ml per animal (4. 5 × 10 8 cfu / ml) plus 2 g of Saccharomyces cerevisiae 9 (7 × 10 cfu/g) (DFM) fed to the animals daily in the morning (Sedighi and Alipour 2019). The diets were adjusted using the Small Ruminants Nutrition Requirements (NRC 2007). The Lambs were fed a fully mixed (TMR) ratio of 30% forage and 70% concentrate at two meals (8 and 16 hours) with free access to water. Ruminal fluid was taken by stomach tube at 0, 3 and 6 hours after morning feeding to measure pH and ammonia nitrogen (NH3-N) concentration. The ruminal fluid was analyzed for ammonia-N using a phenol-hypochlorite method (Broderick and Kang 1980). On the last day of the experiment, rumen fluid was taken to measure the concentration of volatile fatty acids (5 ml of rumen fluid with 2 ml of 25% acid metaphosphoric acid (W/V) was mixed and stored at-20 ° C until analysis). Blood sampling was taken from each lamb within 3 h after the morning feeding from the jugular vein by using EDTA as an anticoagulant. Blood samples were analysed for glucose, blood urea nitrogen (BUN), total protein, cholesterol, triglycerides, LDL, HDL, creatinine and liver enzymes including aspartate amino transaminase (AST) and alanine aminotransferase (ALT). During the last seven days of the period, total faeces and urine samples were collected to determine digestibility and nitrogen retention. Results and discussion: No differences were observed between treatments in dry matter intake (DMI), daily weight gain (ADG), feed conversion ratio, pH and ammonia nitrogen (NH3 – N) (P>0. 05). In the first 21 days of the experiment, body weight gain and average daily gain in the bacterial-yeast recipient treatment were significantly higher than the control (P <0. 05). Observing a lower concentration of ammonia nitrogen at 3 o'clock than 6 o'clock after feed intake is the result of better use of ammonia nitrogen in the rumen due to the greater supply of carbohydrates available in the rumen. Propionate concentration was higher in the bacteria-yeast treatment than other treatments (P <0. 05). Megasphaera elsdenii is the only known rumen microorganism that can convert lactate to propionate by the acrylate pathway; when the lactate concentration increases, which produces propionate and acetate and sometimes butyrate from it (Prabhu et al. 2012). The concentrations of butyrate, isobutyrate, isovalerate and rumen valerate in the bacterial-yeast recipient treatment and bicarbonate Buffer was lower than the control treatment (P <0. 05). The production of branched-chain fatty acids (such as isobutyrate, isovalerate, and valerate) has been shown to be the result of the activity of amylolytic bacteria (Zhang et al. 2017). Protein digestibility was higher in control and bacteria-yeast treatments than in Buffer treatment (P <0. 05). Low-density lipoprotein (LDL) was lower in Buffer and bacteria-yeast treatments than in control treatment (P <0. 05). Nitrogen retention was higher in the bacteria-yeast treatment than in the other treatments (P <0. 05). Significance of nitrogen retention in the bacterial-yeast recipient treatment can be attributed to the decrease in ruminal ammonia nitrogen concentration, which appears to be due to increased nitrogen incorporation in the microbial protein, which is a logical consequence of increased rumen microbial activity (Paryad and Rashidi 2009). Liver factors that are considered as an indicator of liver health were numerically lower in treatments containing Buffer or bacterial-yeast. Conclusion: The use of acid-consuming bacteria can be an effective way to modify rumen fermentation conditions of Lambs fed with high concentrate diets. In the present experiment, the effect of acid-consuming bacteria was competitive with the sodium bicarbonate chemical Buffer on nutrient digestion and growth performance. Results from volatile fatty acids showed that bacteria-yeast treatments by leading the fermentation pathway to convert lactate to propionate could be beneficial for livestock health and its economic longevity.

No record.

APA: Copy

Khorasani, Omid, CHAJI, MORTEZA, & BAGHBAN, FARSHAD. (2020). Comparison of the effect of sodium bicarbonate buffer with Megasphaera elsdenii as a rumen-consuming acid on growth performance, digestibility, rumen, and blood parameters of lambs in high concentrate. ANIMAL SCIENCE RESEARCHES (FACULTY OF AGRICULTURE, UNIVERSITY OF TABRIZ), 30(2 ), 85-99. SID. https://sid.ir/paper/954864/en

Vancouver: Copy

Khorasani Omid, CHAJI MORTEZA, BAGHBAN FARSHAD. Comparison of the effect of sodium bicarbonate buffer with Megasphaera elsdenii as a rumen-consuming acid on growth performance, digestibility, rumen, and blood parameters of lambs in high concentrate. ANIMAL SCIENCE RESEARCHES (FACULTY OF AGRICULTURE, UNIVERSITY OF TABRIZ)[Internet]. 2020;30(2 ):85-99. Available from: https://sid.ir/paper/954864/en

IEEE: Copy

Omid Khorasani, MORTEZA CHAJI, and FARSHAD BAGHBAN, “Comparison of the effect of sodium bicarbonate buffer with Megasphaera elsdenii as a rumen-consuming acid on growth performance, digestibility, rumen, and blood parameters of lambs in high concentrate,” ANIMAL SCIENCE RESEARCHES (FACULTY OF AGRICULTURE, UNIVERSITY OF TABRIZ), vol. 30, no. 2 , pp. 85–99, 2020, [Online]. Available: https://sid.ir/paper/954864/en

No record.

Scientific Information Database

ISSN: 2588-4824

Journal Paper

Comparison of the effect of sodium bicarbonate buffer with Megasphaera elsdenii as a rumen-consuming acid on growth performance, digestibility, rumen, and blood parameters of lambs in high concentrate