ASCOCHYTA RABIEI GENETIC DIVERSITY BY USING OF RAPD STANDARDIZATION

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HOSSEINZADEH KOLAGAR A.; BARZEGAR A.

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Journal: Journal of Agricultural Sciences and Natural Resources Year:2008 | Volume:15 | Issue:1 Page(s): 62-70

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Title

ASCOCHYTA RABIEI GENETIC DIVERSITY BY USING OF RAPD STANDARDIZATION

Author(s)

HOSSEINZADEH KOLAGAR A. | BARZEGAR A. | Issue Writer Certificate

Keywords

DNA POLYMORPHISQ3

ASCOCHYTA RABIEIQ3

RANDOM PRIMERSQ3

BANDS STANDARDIZATIONQ3

Abstract

Ascochyta blight caused by ASCOCHYTA RABIEI (Pass.) Labrousse is a devastaining disease of chick-pea (Cicer arietinum). Fifty-six isolates of ASCOCHYTA RABIEI on chick-pea collected fonm Kermanshah, Hamedan, Lorestan, Kordestan, East Azerbaijan and West Azerbaijan provinces were studied. This isolates were collected from infected chick-pea plants showing the Ascochyta blight symptoms. CTAB method used for genomic DNA extraction from fungal mycelium. Random Amplified Polymorphic DNA/ RAPD studied by 12 RANDOM PRIMERS. Cluster analysis for PCR products of four primers (Ar173, ArPU1, Ar082 and Ar171) performed by UPGMA (Unweighted pair grouped method by arithmatic average) method. Results showed that these isolates because of different patterns of DNA POLYMORPHISm were categorized into seven groups with in 75% similarity levels. We recommend that if the polymorphism of the isolates bands were standardized by migration of marker bands, RAPD technique not only could separate the isolates in multiple groups but also is an effective, rapid, reliable technique to study genetic variability between fungal isolates. Other hand standardization polymorphic bands were used in the similar molecular techniques.

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APA: Copy

HOSSEINZADEH KOLAGAR, A., & BARZEGAR, A.. (2008). ASCOCHYTA RABIEI GENETIC DIVERSITY BY USING OF RAPD STANDARDIZATION. JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, 15(1), 62-70. SID. https://sid.ir/paper/9581/en

Vancouver: Copy

HOSSEINZADEH KOLAGAR A., BARZEGAR A.. ASCOCHYTA RABIEI GENETIC DIVERSITY BY USING OF RAPD STANDARDIZATION. JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES[Internet]. 2008;15(1):62-70. Available from: https://sid.ir/paper/9581/en

IEEE: Copy

A. HOSSEINZADEH KOLAGAR, and A. BARZEGAR, “ASCOCHYTA RABIEI GENETIC DIVERSITY BY USING OF RAPD STANDARDIZATION,” JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, vol. 15, no. 1, pp. 62–70, 2008, [Online]. Available: https://sid.ir/paper/9581/en

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