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Information Journal Paper

Title

Isolation of Newcastle disease virus from industrial poultry and assessing its ability to induce expression of apoptosis-related genes in MCF-7 tumor cells

Pages

  59-71

Abstract

 Newcastle disease virus has been investigated in several studies as an oncolytic virus to treat human Tumors. However, precise mechanism of Tumor cell killing by this virus is unknown. The aim of this study was isolation of Newcastle disease virus from industrial poultry and examination of its ability to induce expression of the extrinsic and the intrinsic pathways of Apoptosis-related genes in human breast carcinoma cells. Newcastle disease virus was isolated from industrial poultry by culture in embryonated chicken eggs and then identified by haemagglutination test and RT-PCR technique. MCF-7 breast carcinoma cells were infected with the isolated virus in vitro. After RNA extraction and cDNA synthesis, Gene expression levels of BAX, BAK, caspases-3,-8, and-9 were assessed in the virus-infected Tumor cells using real-time RT-PCR technique. PCR data analysis showed that Gene expression levels of BAX and caspase-9 were significantly higher in virus-infected Tumor cells than that in control Tumor cells. Gene expression levels of BAK and caspases-3 and-8 were not significantly altered in virus-infected Tumor cells in comparison with control Tumor cells. These results suggest that Newcastle disease virus isolated from industrial poultry can trigger the intrinsic pathway of Apoptosis in MCF-7 Tumor cells.

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    APA: Copy

    Kalantari, Arash, Farashi Bonab, Samad, & Keyvanfar, Hadi. (2020). Isolation of Newcastle disease virus from industrial poultry and assessing its ability to induce expression of apoptosis-related genes in MCF-7 tumor cells. JOURNAL OF VETERINARY MICROBIOLOGY, 16(2 (41) ), 59-71. SID. https://sid.ir/paper/960880/en

    Vancouver: Copy

    Kalantari Arash, Farashi Bonab Samad, Keyvanfar Hadi. Isolation of Newcastle disease virus from industrial poultry and assessing its ability to induce expression of apoptosis-related genes in MCF-7 tumor cells. JOURNAL OF VETERINARY MICROBIOLOGY[Internet]. 2020;16(2 (41) ):59-71. Available from: https://sid.ir/paper/960880/en

    IEEE: Copy

    Arash Kalantari, Samad Farashi Bonab, and Hadi Keyvanfar, “Isolation of Newcastle disease virus from industrial poultry and assessing its ability to induce expression of apoptosis-related genes in MCF-7 tumor cells,” JOURNAL OF VETERINARY MICROBIOLOGY, vol. 16, no. 2 (41) , pp. 59–71, 2020, [Online]. Available: https://sid.ir/paper/960880/en

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