VALIDATION OF A DOMESTIC ELISA AND DETERMINATION OF THE BEST CUT OFF POINT FOR SEROEPIDEMIOLOGICAL STUDY OF ENZOOTIC BOVINE LEUKOSIS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

JAFARI JOZANI R.; MOGHADDAM GH.; JABBARI H.; BAGHERI AMIRI F.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL Year:2012 | Volume:8 | Issue:3 (36) Page(s): 11-16

Download Full-Text

Persian Verion

View:

783

Download:

Cites:

Information Journal Paper

Title

VALIDATION OF A DOMESTIC ELISA AND DETERMINATION OF THE BEST CUT OFF POINT FOR SEROEPIDEMIOLOGICAL STUDY OF ENZOOTIC BOVINE LEUKOSIS

Author(s)

JAFARI JOZANI R. | MOGHADDAM GH. | JABBARI H. | BAGHERI AMIRI F. | Issue Writer Certificate

Keywords

ELISAQ2

RELATIVE SENSITIVITYQ2

BOVINE LEUKEMIA VIRUS (BLV.)Q2

RELATIVE SPECIFITYQ2

Abstract

The aim of this study was to compare the validation of a Domestic Indirect-ELISA with a Commercial Indirect-ELISA in order to find the optimum cut off value for detection of antibodies to Bovine Leukemia Virus (BLV). BLV proteins harvested easily as described by OIE and used in DI-ELISA. A domestic ELISA was adapted by checker board methods. 460 sera from different herds were applied in the commercial ELISA as a reference test and then compared with the domestic ELISA. All the negative control sera except one (E05 serum) were chosen by commercial ELISA. Statistical analysis of negative sera showed that consideration of a cut off value, from 0.26 to 0.30 will provide acceptable RELATIVE SPECIFITY, RELATIVE SENSITIVITY and can reducing the risk of false positive results for Domestic Indirect ELISA. The best RELATIVE SENSITIVITY (86%) and specifity (88%) was yielded in 0.27 optical density (OD).As calculated, frequency of seropositive animals was 8% and 10% by domestic and commercial ELISA respectively.

Cites

No record.

References

No record.

Cite

APA: Copy

JAFARI JOZANI, R., MOGHADDAM, GH., JABBARI, H., & BAGHERI AMIRI, F.. (2012). VALIDATION OF A DOMESTIC ELISA AND DETERMINATION OF THE BEST CUT OFF POINT FOR SEROEPIDEMIOLOGICAL STUDY OF ENZOOTIC BOVINE LEUKOSIS. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 8(3 (36)), 11-16. SID. https://sid.ir/paper/97160/en

Vancouver: Copy

JAFARI JOZANI R., MOGHADDAM GH., JABBARI H., BAGHERI AMIRI F.. VALIDATION OF A DOMESTIC ELISA AND DETERMINATION OF THE BEST CUT OFF POINT FOR SEROEPIDEMIOLOGICAL STUDY OF ENZOOTIC BOVINE LEUKOSIS. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL[Internet]. 2012;8(3 (36)):11-16. Available from: https://sid.ir/paper/97160/en

IEEE: Copy

R. JAFARI JOZANI, GH. MOGHADDAM, H. JABBARI, and F. BAGHERI AMIRI, “VALIDATION OF A DOMESTIC ELISA AND DETERMINATION OF THE BEST CUT OFF POINT FOR SEROEPIDEMIOLOGICAL STUDY OF ENZOOTIC BOVINE LEUKOSIS,” SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, vol. 8, no. 3 (36), pp. 11–16, 2012, [Online]. Available: https://sid.ir/paper/97160/en

Related Journal Papers