CLONING AND EXPRESSION OF VP28 GENE OF WHITE SPOT VIRUS (IRAN ISOLATE) IN E. COLI, TG1

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HOUSHMAND H.; SEYFI ABAD SHAPOURI M.R.; PEYGHAN R.; GHOFLEH MARAMAZI J.; AFSHARNASAB M.

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Journal: SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL Year:2015 | Volume:11 | Issue:1 Page(s): 110-119

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Title

CLONING AND EXPRESSION OF VP28 GENE OF WHITE SPOT VIRUS (IRAN ISOLATE) IN E. COLI, TG1

Author(s)

Keywords

WHITE SPOT VIRUSQ2

VP28Q2

CLONINGQ2

EXPRESSIONQ2

Abstract

WHITE SPOT VIRUS is concerned as a major pathogen in shrimp Penaeids family. In recent years, heavy mortalities in cultured shrimps Iran and other countries necessitate doing studies to construct kits for rapid detection and eliciting resistance against the virus by using different membrane proteins. For this purpose, in this study, a piece of VP28 gene of WHITE SPOT VIRUS with 528 bp length was isolated and amplified by PCR from the infected shrimps in Choebdeh Abadan. After CLONING of the gene in the plasmid pMal-c2X, the protein was expressed inE.colistrain TG1. Target protein with a molecular weight of 19.38 kDa was evaluated by SDS-PAGE. EXPRESSION of protein with the expected molecular weight in SDS-PAGE accompanied with evaluation of the cloned sequences revealed the successful EXPRESSION of the protein inEscherichia coli TG1. This study for the first time succeeded in CLONING and experssion of VP28 in a prokaryotic organism.

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APA: Copy

HOUSHMAND, H., SEYFI ABAD SHAPOURI, M.R., PEYGHAN, R., GHOFLEH MARAMAZI, J., & AFSHARNASAB, M.. (2015). CLONING AND EXPRESSION OF VP28 GENE OF WHITE SPOT VIRUS (IRAN ISOLATE) IN E. COLI, TG1. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 11(1), 110-119. SID. https://sid.ir/paper/97435/en

Vancouver: Copy

HOUSHMAND H., SEYFI ABAD SHAPOURI M.R., PEYGHAN R., GHOFLEH MARAMAZI J., AFSHARNASAB M.. CLONING AND EXPRESSION OF VP28 GENE OF WHITE SPOT VIRUS (IRAN ISOLATE) IN E. COLI, TG1. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL[Internet]. 2015;11(1):110-119. Available from: https://sid.ir/paper/97435/en

IEEE: Copy

H. HOUSHMAND, M.R. SEYFI ABAD SHAPOURI, R. PEYGHAN, J. GHOFLEH MARAMAZI, and M. AFSHARNASAB, “CLONING AND EXPRESSION OF VP28 GENE OF WHITE SPOT VIRUS (IRAN ISOLATE) IN E. COLI, TG1,” SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, vol. 11, no. 1, pp. 110–119, 2015, [Online]. Available: https://sid.ir/paper/97435/en

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