An 8-year-old crossbred thoroughbred chestnut stallion was suspected to an autoimmune mediated skin disease based on history, clinical observations, microbiological and clinicopathologically findings. The clinical signs disappeared completely after five weeks of treatment with PO prednisolone. However, approximately eight months later, the lesions recurred in a more severe form. There was no improvement despite the repeating previous treatment by horse's owner; therefore, oral azathioprine was added to prednisolone after skin biopsy. A significant therapeutic effect was observed by this combination, however; the use of azathioprine was discontinued after fifteen days due to diarrhea, while prednisolone continued for another week. After improvement of the symptoms, oral prednisolone was also stopped with a decreasing trend. Vesiculobullous to pustular lesions with acantholytic characteristic of the stratum corneum and stratum granulosum of epidermis were the histopathological findings, characteristics of pemphigus foliaceus. This is the first reported case of recurrent pemphigus foliaceus in Iran.

Introduction: Non- steroidal anti-inflammatory drugs (NSAIDs) are drugs commonly prescribed in dental practice for the management of pain and swelling. But, rarely hypersensitivity reactions are reported.Case Report: A 28 year old woman underwent periodontal plastic surgery (gingival graft). Postoperative analgesics (400 mg Gelofen, oral) and antibiotics were administrated for the patient. Three hours after discharge of patient, she complained of redness, itching, rapid swelling of her eyes in 10 minutes, and watery eye discharge 1 hour after taking the drugs. She was treated with 8mg/2ml mg Dexamethasone IM at the dental department and with Hydrocortisone 100mg/ml IM and antihistamine drugs at the hospital.Conclusion: There are no published protocols and sensitivity and specifity of skin pick testing and patch testing for Gelofen. So avoidance of re- exposure is the best management strategy. The use of Cox-2 specific medications would be a proper alternative for pain relief.

Introduction: The aim of the present study was to investigate psychological well-being and Sensory processing sensitivity levels based on the change in the circadian cycle Morningness and eveningness. Method: For this purpose, Initially 475 students of Urmia were selected, in the form multistage cluster sampling, Then Morningness and eveningness types Questionnaire In order to responding were distributed among them. After analysing the initial results, 51 participants were selected for the second stage According to obtained scores in personality traits questionnaire Morningness and eveningness, as individuals with Morningness and eveningness and intermediate. Finally, , in order to test the hypothesis of this study, participants were have studied to measure well-being and processing sensitivity, Psychological well-being scale and scale of individual with sensory processing sensitivity were distributed among participants in the study in order to collect data. Collected data were analyzed to investigate the research goals. Result: Results indicated significant differences between dimensions of Psychological well-being based on the types of Morningness and eveningness and intermediate, Morningness types had a lower Psychological well-being, also comparison of Morningness and eveningness in Sensory processing sensitivity Dimensions, showed that groups of Morningness types had higher sensitivity to stimulus. Discussion and conclusion: It seems that individual differences in circadian cycles, may be related to levels of mental health and lead to different reactivity to stimulants.

Background: Application of continuous cell lines has got a special place in the virological researches. These cells are immortal and their chromosomes are aneuploid. Therefore, they can be passage without any limitation. The aim of this research was to choose the best way of producing clone of cells. Methods: in this study, Bovine Kidney (BK) cell line was used to be cloned through limiting dilution method in which Vero cells were used as feeder layer. Vero cells were first cultured in DMEM supplimented with 7% heat inactivated calf serum and after a monolayer were formed, their growth was arrested by Mitomycin C. The cloned cells after incubation were separated and cultured in a new flask. After several experiments different clones were obtained and cultured for further studies. Results: Karyotype of clone cells were determined and compared with original cells. It was shown that cloned cells were more homogenous in early passages and their karyotypes showed less variability than original ones. Cloned and original cells were inoculated with HSV-1 and Parainfluenza virus 3 in order to evaluate its biological abilities. Tissue culture of infectious dose 50 (TCID50) of each virus was calculated and it was shown that there was no significant different between the HSV-1 titers before and after cloning whereas the titer of the Parainfluenza virus 3 was significantly higher in the original cells. Conclusions: Cloned cells of BK showed more stable karyotype and were less sensitive to parainfluenza type-3 virus infection than original BK cells.

Introduction: Many clinical studies have evaluated different dentin bonding agents in treatment of dentin hypersensitivity (DH). But the use of acid is controversial and the effect of self-etch bonding agents isn't clearly defined. Objective: The aim of this study was to compare two dentin bonding systems, prompt (self-etch bonding system) and excite(with and without acid) in treatment of DH.Materials and Methods: This clinical study had a double-blind, randomized, subject design and was performed on 41 sensitive teeth with DH. We had 3 groups e1 (we used excite without acid), e2 (excite with acid) and p (prompt). Two stimuli (cold water 20‎ْc and compressed air) and two methods for evaluation ((VAS (Visual Analogue Scale) and response time)) in 3 times (before treatment, immediately after treatment and two weeks after it) were used.Results: All treatments showed significant reductions in DH. In the VAS scores, no significant difference was found. But in the response time, the groups e1, P significantly showed better results than group e2.(P<0.05)Conclusion: Treatment effects were improved in all groups with time. Use of acid before the bonding agent had a negative effect but there was no significant difference between prompt and excite (without acid etching). It's worth nothing that the measurement of response time to stimulus compared to the VAS method is more accurate in evaluation studies of pain rate.

Background and Aims: Cervical tooth hypersensitivity (CTH) is defined as a sharp pain of short term that occurs in dentin exposed to the oral environment. The aim of this study was to evaluate the efficacy of a desensitizing toothpaste containing potassium nitrate (Sensodyne®) for relieving of CTH, when used locally or as tooth paste.Materials and Methods: 36 patients were enrolled in this study. They were randomly divided into three groups I) Tooth brushing without toothpaste, II) Local application of toothpaste containing potassium nitrate 5%, III) Tooth brushing with toothpaste containing potassium nitrate 5%. Assessment of CTH was done by evaporative (using air and water spray) and cold stimuli at baseline, 1, 3, and six weeks after the treatment. To compare pain changes over time, due to the interaction between the variables of the studied groups and time, one-way ANOVA was used at any time. Due to the difference of variance between groups to compare pairs of groups, Dunnett T3 test was used.Results: During the study period, no adverse event observed. In all three groups, pain decreased gradually. Local application of toothpaste had the most favorable effect on CTH. Therefore, when compared with that of the first group, the percentage reduction in pain were statistically significant (P=0.03).Conclusion: Local application of toothpaste containing potassium nitrate as a desensitizing ingredient is a better choice for treatment of CTH than that of application with toothbrush.