Eucalyptus trees are fast-growing trees and some of species are tolerance to un-favorite conditions. Seedling production is a pre-condition for agroforestry in suitable regions of Iran. Micropropagation is a useful tool for asexaul production. Conventional micropropagation is an expensive method, because of high rate contamination and death of plantlets during transferring to soil and using high amount of biochemical plant growth regulaturs. Semiphotoautotrophic system is used because of omitting sucrose in the media, using exogenesis growth regulators in low amount or not using at all, and better rooting and shooting of plantlets. So this system decrease the expenses of in vitro plantlets production. This work, was studied the effects of G7 and B.F.J with and without filtered cap. Growth indicators,i.e. root fresh yield, root dry yield, shoot fresh yield, shoot dry yield, leaf surface, leaf number, root length, shoot length, and shoot number, of three difference species Eucalyptus camaldulensis, E. melliodora and E. micrpcarpa were survayed in semiphotoautotrophic conditions. The G7 filtered cap vessels were better than the other for growth indicators which were studied. The range of survival for transferring seedling to soil was 100% for E. melliodora and E. micrpcarpa species and 65% for E. camaldulensis. Semiphotautotrophic not only reduce the micropropagation costs, but also improve the growth indicators. However, its problems, i.e. surface and internal contamination of explants, phenolic components release and etc. should be considered in semephotoautotrophic micropropagation system.

People living with cystic fibrosis (CF) are believed to perform poorly at high altitude. The advent of cystic fibrosis transmembrane conductance regulator (CFTR) modulators has transformed patient care, making high-altitude trekking an attainable goal for most patients living with CF. We consider how an amateur trekker with CF who wishes to climb a high mountain such as Kilimanjaro (5895m) should be advised. Since symptomatic pulmonary hypertension is a contraindication to high altitude travel, CF patients with pulmonary hypertension should use supplemental oxygen and take nifedipine for HAPE prophylaxis. There is no contraindication to the use of prophylactic acetazolamide in CF. With the success of CFTR modulators, it may no longer be necessary to optimise the CF patient’, s clinical status with an inpatient course of intravenous antibiotics in advance of high-altitude travel. High-altitude trekkers with CF should use volumatic spacers with their inhalers and portable nebulisers. They should continue to take their regular medications, including mucolytic agents and prophylactic oral antibiotics. Monitoring oxygen saturation using a pulse oximeter may be useful. On the Kilimanjaro trek, a longer climbing route such as Lemosho is preferred as it allows more time for ACCLIMATISATION to hypoxia. A formal medical fitness-to-travel assessment by a respiratory physician may be mandated by insurance companies. Close liaison between respiratory and travel medicine specialists will best serve the needs of high-altitude trekkers with CF.