FT-SERS (Fourier Transform Surface Enhanced Raman Scattering) of Adriamycin and its complex with DNA is reported. It is shown that in agreement with previous Raman studies the interaction of Adriamycin with DNA takes place through an intercalation mechanism. The presence of a new band at 731 cm-1 suggests that ring D of Adriamycin is not involved in the intercalation process.

In this study calf thymus chromatin was fractionated into active (S1and S2)and inactive (P2) chromatin. Then the interaction of an anthracycline antibiotic, Adriamycin, with these fractions was investigated employing absorption and difference UV/Vis spectroscopy and SDS and agarose gel electrophoreses. The results suggest that the binding of Adriamycin to the S1 fraction is slight but S2and P2 were considerably affected by drug action. In both cases the absorbances at 260 and 480 nm were decreased. As drug concentration is increased in the mixture, core histones are aggregated and coprecipitate with the chromatin. The extent of condensation in P2 is higher when compared to the S2fraction.

The aim of this study is to evaluate the effects of pravastatin on Adriamycin (ADR) -induced nephropathy and the mechanisms involved. Forty rats were divided into the following 4 groups: control, ADR (15 mg.kg-1, IP), ADR plus pravastatin (20 mg.kg-1 which was started 5 days prior to ADR injection), and ADR plus pravastatin (20 mg.kg-1 which was started 5 days after ADR injection). On day 20 after ADR injection, the animals were sacrificed. The results showed that administration of pravastatin decreased the levels of 24-h urinary protein (24-h UP), blood urea nitrogen (BUN), and creatinine (p<0.05) which had increased after the injection of ADR; in addition, pravastatin reversed structural changes seen in ADR group. Furthermore, pravastatin elevated mRNA and protein expression of nephrin (p<0.05) which had been reduced in ADR group. We conclude that pravastatin protects and treats renal injury induced by ADR.