In order to evaluation of genetic variation in segregation population (307 F5-plants), which are upshot of mating of Azar2 with 87-Zhong291 cultivars, we planted these lines in Augmented design with four check varieties (Toos, Shahriyar, Sardari and Azar2 and 87-Zhong291 line). These check varieties planted in RCBD design with 6 replication and lines sat between these check varieties in the separated rows, we used toos check varieties as a general check in the first, middle and last replication. For germination of grains, we irrigated the farm just one time for sprouting. Within and end of growth season we measured some agronomic and morphological characters in both vegetative and reproductive stages such as Plant height, Grain yield, Spikelet accumulation etc. After adjusting the data, according to Stepwise regression around of 50 percent of variety between genotypes for Seed yield result of traits variety such as Biological yield, Number of seed in main spike and Day to flowring (except Harvest index as a predictors). According to multiple statistics methods like Factor analysis recognizing seven factores such as Seed yield, Plant height, Length of peduncle, Spikelet accumulation, Harvest index, Weight of 100 seed and Number of mature tiller. Cluster analysis with using of mean of datas method had done and at last 106 variable lines in 14 groups confirmed and finally 4 most grain yield had selected.