Background: Avocado PEELs (Persea americana Mill.) contain polyphenolic compounds that can be used as alternative medicinal plants, such as anti-inflammatory, antibacterial, and antifungal agents. This research aims to determine the acute toxicity of ethanol extract from avocado PEELs on ddY strain mice.
Methods: This experimental research using Acute Toxic Class Method (OECD 423) was conducted on 15 female mice ddY strain that were divided into five groups with one control group (CG) and four treatment groups. The four treatment groups administrated by ethanolic extract of avocado PEEL at Group 1 (G1) with 300 mg/kgBW single dose, Group 2 (G2) with 300 mg/kgBW repeated dose, Group 3 (G3) with 2,000 mg/kgBW single dose, and Group 4 (G4) with 2,000 mg/kgBW repeated dose were observed for 14 days.
Results: The results showed that avocado PEELs did not cause any toxic symptoms, including mortality (0%). No behavioral abnormalities or macroscopic changes in organ color, size, and relative organ weight were observed. Statistical analysis revealed no significant differences in the relative liver and kidney weight between the control and treatment groups (P>0.05). Although a reduction in body weight was noted, it was not statistically significant (P>0.05)
Conclusion: All doses of the ethanolic extract of avocado PEELs fall into the practically non-toxic category (category 5) based on Globally Harmonised System (GHS).