Obesity is a global health concern leading to various chronic diseases, including alterations in liver enzymes. This study aimed to investigate the effect of TRX training program on liver enzymes in obese middle-aged women. In this quasi-experimental study, 30 obese women with a BMI above 30 and an average age of 50. 00 ± 6. 87 years were randomly divided into two groups: TRX (n=15) and control (n=15). The TRX group engaged in TRX training for eight weeks, while the control group maintained their usual lifestyle. Blood samples were collected before and after the intervention to measure liver enzymes. Anthropometric measurements were also taken before and after the intervention. Data were analyzed using ANCOVA to control for pre-test differences and determine post-test differences between groups. The ANCOVA results revealed significant differences in post-test liver enzyme levels between the TRX and control groups, after controlling for pre-test scores. For ALT, F(1, 27) = 12. 94, p = 0. 001, partial eta squared = 0. 324,for AST, F(1, 27) = 10. 12, p = 0. 003, partial eta squared = 0. 273,and for ALP, F(1, 27) = 25. 62, p < 0. 001, partial eta squared = 0. 487, indicating significant improvements in the TRX group compared to the control group. This study demonstrated that eight weeks of TRX training significantly improved liver enzymes ALT, AST, and ALP in obese women. Therefore, TRX training can be an effective intervention for enhancing liver enzyme health.