Introduction: Cattle are a monotocous species meaning that, under most circumstances, a successful pregnancy results in the birth of one calf. TWINNING rate has been reported in dairy cows from 3 to 5 percent, which can be influenced by maternal age. The birth of twins is detrimental to the majority of beef and dairy cattle producer. Financial loss arising from any of TWINNING has been reported in Europe between 109 to 201 dollars in recent years. Because it is associated with undesirable consequences such as reduced survival, calf, cow increased removal rate and poor performance. This also reduces pregnancy rates and profitability herds. One of the effects of TWINNING severe is reduction of the number of calves for replacement fertility in dairy cows. This is a loss arising from an increase in infant mortality and a gender bias in bull calves homo zygote. TWINNING rate increases significantly the incidence of reproductive abnormalities, including the retained placenta, dystocia, stillbirth and abortion. Many studies have been done on the effect of multiple pregnancies in cattle production and reproduction. Higher milk production for cows twin issue is controversial as some studies have shown that there is a positive correlation between the rate of TWINNING in dairy cattle and milk production. But in the next lactation, production for cows that have been the twin of the infected cow metabolic disease in the previous period was lower. In a study reported that cows spend fewer days in the twin peak production. The results of the study on the effect of TWINNING on reproductive traits of Holstein cows-Farzin showed that only half of the twin cows are prone to reproduce in the next period. It is also reported a greater number of insemination per conception in twin compared to single cows. In addition, it has been reported that the twin was more than 15 days from calving to first services. Average twin cows experiencing 1.7 times more death and removal in lifetime production than cows single. The aim of this study was to estimate the rate of TWINNING in Iranian Holstein dairy cows and the estimated effect on production traits and reproductive TWINNING....