Desert as a biome of biosphere, resulted from atmospheric cyclones. It is obviously accepted by anyone, that desertification phenomenon, which is acquired under climate change and insufficient development of human activities, threaten environment. Without considering amount of efficiency of these factors on desertification, what it were not regarded under conditions our country, determination of real desert domain based on different environmental factors. At this paper, we tried to map the desert regions of Khorasan province using climatic factors. In order to reach the results, firstly, meteorological stations net of Khorasan, were identified and 85 station were selected. The local distribution of these stations as a point map was plotted using Ilwis software. Recent layer was used as a base map for other works. Continually, some of climatic factors for example coefficient of variation of precipitation, Demartonne aridity index, isohytes, isotherms, and etc, which reflected climatic characteristics of desert regions, calculated and analyzed for any station. Then, isometric maps of each climatic factor plotted using Surfer software. Finally different layers overlaid together using Geographical Information System and three zones released on final map. Inter zone with harsh conditions was contained real or natural deserts with over of 22 million hectare area and middle zone which is named Semi-desert, included desert and non-desert characteristics. Results showed that environmental factors varied gradually and desertification processes, were active on transition zone.