Chromosome number and karyotype analysis of five monocot mangrove associates from Dhamra and Paradeep coast of Orissa revealed 2n=48 in Porteresia coarctata; 2n=22 in Crinum defixum and Cryptocoryne ciliata and 2n=20 in Asparagus racemosus var. javanica and Cyperus cephalotes. Total chromosome length varied from 42.44mm in A. racemosus var. javanica to 93.86mm in C. defixum. Significant variations of chromosome volumes were also recorded among the species. The 4C DNA content varied significantly from 47.15pg in C. defixum to 6.31pg in C. cephalotes. A positive significant correlation between the chromosome volume and 4C nuclear DNA content among five different species was revealed. The genome size was around 1,545mbp in C. cephalotes to 4,074mbp in P. coarctata with a highest size (11,551mbp) in C. defixum that suggests the high repetitive DNA sequences in the Crinum with highest chromosome length and volume of this species.