Swallow-wort, is a perennial invasive twining of Milkweed family that has become problematic in many parts of Iran, especially in orchards. Present study was conducted in order to study the effect of temperature on asexual reproduction ability of this weed. The following experiments were conducted in Iranain Research Institute of Plant Protection during 2003-2004: 1- Effect of temperature on root asexual proliferation with 3 factors and 4 replications that the first factor (A) was duration (0 (control), 24, 48, 96 and 192 hrs) of root keeping in oven; the second factor (B) was root length (3, 6, 9 and 12 cm), and the third factor (C) was oven temperature (10, 20 and 30oC). 2- Effect of freezing on root asexual proliferation with 3 factors and 4 replications that the first factor (A) was duration (0(control), 24, 48, 96 and 192 hrs) of root keeping in freezing, the second factor (B) was root length (3, 6, 9 and 12 cm) and the third factor (C) was freezing temperature (-1, -3 and -50C). Under 20 and 30.C temperatures, the roots were completely destroyed, but 10oC for 24, 48 and 96 hours could not kill all the roots. Freezing temperatures of -50C or -3oC for 96 hours killed all the roots. Freezing temperatures of -3oC for up to 48 hours, or -1oC, could not completely kill the roots. Therefore, it seems that desiccation and freezing of roots are suitable approaches for management of swallow-wort.