Objective: There are still worldwide controversial attitudes regarding embryo donation (ED) program. The aim of this descriptive study was to evaluate the infertile couples' opinions regarding ED program for infertility treatment in Iran.Methods: A total of 120 infertile patients (women, n=6l; men, n=59) residing in Iran were asked to fill out the questionnaires. Part I of the questionnaire form contained general demographic information. Part II contained 17 questions to reveal their knowledge and attitudes about ED program in Iran.Results: The vast majority of the subjects had formal education. One-third had complete objection toward ED. 21% and 14% of the women and men thought that ED is not permitted by their religion, respectively. Nearly half of participants believed in informing the general public about ED in mass media. In addition, majority of the participants were in favor of ED over adopting a child. Psychological counseling was strongly recommended by vast majority of respondents for both donor and the recipient of the embryos.Conclusion: The vast majority of infertile patients had positive attitudes toward ED as treatment for selected infertile cases. However, their knowledge on their religious attitudes was limited. Therefore, the mass media and healthcare professional should develop programs for informing the public regarding the ED.