Introduction: It can be said that many of the human researches are followed after animal testing’s. But the researchers should be concerned about animal legal rights and the ethical animal research.Material and Methods: In a descriptive study in 11 research centers in Tehran University of medical sciences were evaluated considering housing, feeding, care and use of the laboratory animals as well as the knowledge, attitude and practice of researchers and animal care givers. The results were analyzed using Spss software.Results: In this study, 29 researchers and 10 personnel evolved in animal care were evaluated. Most of the researchers had the high score of knowledge, attitude and practice in choosing the adequate species and numbers of the animals to obtain valid results also knowing the signs of stress and pain in them, but they had poor knowledge about animals' appropriate environmental conditions, breeding, breast feeding, and euthanasia and emit of animal bodi's. Only 40% of animal care givers knew their important role in animal health and its influence on the accuracy of the investigations on them as the result. 60% of them had enough information on animal disease, breeding and light and dark cycles in their environment but their attitude and practice was not enough. 60% of them didn't know human killing methods of animal, preventing methods of transmission of zeonosis. 50% of animal care givers didn't know about emit of animal bodi's but 80% of them used one of methods for that. Their room or cage condition, food, water and cage sanitation were at a standard condition.Conclusion: According to the result, it seems that general procedure including standardization of their room, specialty control of environmental condition and providing the capability of emitting of animal bodi's should be applied by animal care providers. Also for safe living, care and use of the laboratory animals it is necessary to provide appropriate training for the research staffs and animal care givers.